The Effect of Nitroglycerin in Testicular Histopathology, Reproductive Hormones and Testicular Oxidative Stress in Adolescent Rats

Koşal, Volkan; Okulmuş, &Ccedil;ağlar; Ko&ccedil;ak, Yılmaz; Keleş, &Ouml;mer Faruk; Başbuğan, Yıldıray; Ceylan, Nesrin

doi:10.21608/mvmj.2023.228652.1146

The Effect of Nitroglycerin in Testicular Histopathology, Reproductive Hormones and Testicular Oxidative Stress in Adolescent Rats

Document Type : Original Articles

Authors

¹ Department of Artificial Insemination, Faculty of Veterinary, Van Yuzuncu Yil University, Van, Turkey

² Department of Biochemistry, Izmir/Bornova Veterinary Control Institute, TR-35030 Izmir, TURKEY

³ Physical Therapy and Rehabilitation, Faculty of Health Sciences, Van Yuzuncu Yil University, TR-65080, Van, Turkey

⁴ Department of Pathology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, TR-65080, Van, TURKEY

⁵ Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yıl University, TR-65080, Van, TURKEY

⁶ Department of Pediatric Neurology University of Health Sciences, Dr. Sami Ulus Maternity and Children’s Health and Diseases Training and Research Hospital, TR-06080, Ankara, Turkey

10.21608/mvmj.2023.228652.1146

Abstract

Objective: Nitroglycerin (NTG) demonstrates a vasodilation effect by acting on vascular smooth muscles. The exact impact of vasodilation on the testes is not fully understood. The aim of this study is to investigate the effects of NTG on the testes before the onset of spermatogenesis and reproductive activities in adolescent rats.

Animals: Average of 45 days old male Albino Wistar rats were used.

Design: Two different groups were formed: an adolescent control group and an adolescent NTG group (10mg/kg, 5 days).

Results: At the end of the study, testicular oxidative stress (MDA, CAT, AOPP, NO), reproductive hormones (FSH, LH, Testosterone), and testicular histopathology were examined. In comparison to the control group, the testicular tissue MDA and NO levels in the NTG group were significantly increased (p<0.05), while CAT activity was decreased (p<0.05). Upon examining reproductive hormone levels, an increase in FSH and LH was observed in the NTG group (p<0.05), while testosterone levels remained unchanged. Histopathological examination revealed the onset of changes in the tubulus seminiferus contortus of NTG group rats.

Conclusion and clinical relevance: As a result of this study, it was determined that the use of NTG in adolescent male rats increases testicular oxidative stress and affects reproductive hormone synthesis.

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