Evaluating the potential use of resveratrol in alleviating biochemical alterations, immune responses, and tissue pathology-associated with subacute malathion toxicity in Nile tilapia.

Document Type : Original Articles


1 Veterinarian at the Veterinary Administration in EL Mahalla EL-Kubra,Egypt

2 Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University, Egypt


Objective: To study the  use of Nile tilapia as an aquatic model to investigate the biochemical, immunological, and pathological alterations after exposure to subacute MLT toxicity, and to evaluate the potential use of resveratrol (RES) as a dietary supplement to protect against MLT-induced toxicity.
Design: Randomized controlled study.
Fish:   180 apparently healthy Oreochromis niloticus .
Procedures: Four experimental groups of 45 fish each were performed: the control group received only tap water free of MLT; the MLT group was intoxicated with MLT (1/5 of the LC50 of MLT); the RES group was exposed to water free of MLT and given a RES-supplemented diet (50 mg/kg of the diet); and the MLT- RES group was exposed to MLT (1/5 of the LC50 of MLT) and RES (50 mg/kg of the diet). The experiment was extended for 4 weeks.
Results :The results indicated marked increases in ALT, AST, creatinine (Cr), and urea (Ur), along with a considerable decrease in serum total proteins (TP) and albumin (Alb) in the MLT group compared to other groups. Besides, there were increases in nitric oxide (NO) and malondialdehyde (MDA) levels, as well as decreases in catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), and reduced-glutathione (GSH) in the liver, kidney, and gill tissues of the MLT group compared to other groups. Also, up-regulation of interleukin-1β (IL-1β), interleukin-8 (IL-8), and transforming growth factor-1β (TGF-1β) in the liver and anterior kidney of MLT group compared to other groups. Finally, alterations in liver, intestine, and gill tissues associated with oxidative stress damages in the MLT group compared to other groups.
Conclusion and clinical relevance: RES treatment helped to alleviate the MLT-induced toxicity since it possesses potent antioxidant and anti-inflammatory effects.


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