Document Type : Original Articles
Theriogenology department, faculty of veterinary medicine, Mansoura, Egypt
Department of Theriogenology, Center for Reproduction Biology, Faculty of Veterinary Medicine, Mansoura, University, Egypt
Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research &lrm;Institute, Al Haram, Giza, Egypt&lrm;
Theriogenology Department, Center for Reproduction Biology, Faculty of veterinary Medicine, Mansoura University,
Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
Theriogenology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Physiology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt
Mansoura University, Faculty of veterinary Medicine
The objective: Proteins and other nitrogenous substances such as polyamines, catecholamines, polyamines, and nitric oxide require amino acids for synthesis. The purpose of this study was to see how adding l arginine to either maturation or vitrification medium affected the developmental competence of immature buffalo oocytes.
Design: Randomized controlled experimental study
Study Design: In this study, different concentrations of l arginine (50, 100, and 250 ul/ml) were used. The results were consistent for both maturation and vitrification.
Results: When compared to other concentrations (p˂0.05) and the control group, the recovery rate of immature buffalo oocytes after supplementation with L arginine (L Arg) in different concentrations was lower when using 150ul of L Arg (70.8 percent). When the l arginine concentration was 250 ul/ml, however, the rate of cleavage was higher (p˂0.05) when compared to other concentrations and control (p˂0.05). The percentage of morula was on the same line as the cleavage rate. When compared to control and the other two L Arg concentrations (0.05), the results showed a higher morula rate (p˂0.05) associated with supplementation of media with a higher concentration of L arg (250ul/ml).
Conclusion: Finally, the addition of maturation as well as vitrification media reduces the developmental competence of mature and immature buffalo oocytes. Further research into the signaling pathway for l arginine in buffalo oocytes may be required.