Document Type : Original Articles
Authors
1
Theriogenology department, faculty of veterinary medicine, Mansoura, Egypt
2
Department of Theriogenology, Center for Reproduction Biology, Faculty of Veterinary Medicine, Mansoura, University, Egypt
3
Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research ‎Institute, Al Haram, Giza, Egypt‎
4
Theriogenology Department, Center for Reproduction Biology, Faculty of veterinary Medicine, Mansoura University,
5
Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
6
Theriogenology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
7
Physiology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt
8
Mansoura University, Faculty of veterinary Medicine
Abstract
The objective: Proteins and other nitrogenous substances such as polyamines, catecholamines, polyamines, and nitric oxide require amino acids for synthesis. The purpose of this study was to see how adding l arginine to either maturation or vitrification medium affected the developmental competence of immature buffalo oocytes.
Design: Randomized controlled experimental study
Study Design: In this study, different concentrations of l arginine (50, 100, and 250 ul/ml) were used. The results were consistent for both maturation and vitrification.
Results: When compared to other concentrations (p˂0.05) and the control group, the recovery rate of immature buffalo oocytes after supplementation with L arginine (L Arg) in different concentrations was lower when using 150ul of L Arg (70.8 percent). When the l arginine concentration was 250 ul/ml, however, the rate of cleavage was higher (p˂0.05) when compared to other concentrations and control (p˂0.05). The percentage of morula was on the same line as the cleavage rate. When compared to control and the other two L Arg concentrations (0.05), the results showed a higher morula rate (p˂0.05) associated with supplementation of media with a higher concentration of L arg (250ul/ml).
Conclusion: Finally, the addition of maturation as well as vitrification media reduces the developmental competence of mature and immature buffalo oocytes. Further research into the signaling pathway for l arginine in buffalo oocytes may be required.
Keywords
Main Subjects
)