Efficacy of Adipose Tissue and Bone Marrow Derived Mesenchymal Stem Cells for Treatment of Surgically Induced Knee Osteoarthritis in Rats

Document Type : Original Articles


1 Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Egypt.

2 Department of Toxicology, Faculty of Medicine, Mansoura University,Egypt.

3 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Egypt.

4 Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Egypt.


Objective: to evaluate the efficacy of direct intra articular (IA) injection of adipose tissue (ADSCs) and bone marrow (BMSCs) derived mesenchymal stem cells for regenerating articular cartilage in surgically induced knee osteoarthritis (OA) in sprague-dawely rats.
Design: A randomized-controlled study.
Animals: thirty Sprague dawely rats were divided into 5 groups (6 rats in each group).
Procedures: OA was surgically induced by medial collateral ligament (MCL) transection and medial meniscal tear of the leftt knee joints to induce joint destabilization of the left knee. 4 weeks after the operation, single dose of ADSCs, BMSCs and phosphate buffer saline (PBS) was delivered to the operated knee by direct aseptic IA injection in each treated group(III), (IV) and (V)respectively. The healing was assessed clinically, grossly and microscopically at 10 weeks postoperative.
Results: rats that received ADSCs grossly showed better efficiency in regenerating of articular surface and lower degree of cartilage degeneration histopathologically and there was a trend towards higher scores for all parameters in treated groups, and demonstrated that chondrocytes and chondroid matrix regained their histological architecture upon ADSCs more than BMSCs.ADSCs is mimicking the normal articular cartilage, the BMSCs alleviated inflammatory process and increased the lay down of chondroid matrix. ADSCs exhibited mild Immunohistochemical expression against Matrix Metalloproteinase 9(MMP9) in comparison to other treated groups
Conclusion and clinical relevance:
the ADSCs and BMSCs have a potent anti-inflammatory, strong regenerative capacity on chondrocytes and increased chondroid matrix deposition. ADSCs is more effective and easily available than BMSCs


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