Anaerobic spore forming bacteria contaminating some vacuum packaged meat products

Document Type : Original Articles

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Abstract

The present study was designed to determine the anaerobic spore forming bac­ teria contaminating some vacuum packaged meat products. A total of 45samples of Cocktail , Salami and Pepperoni (15 of each) were collected randomly from different su­ permarkets in Alexandria governorate. The obtained results revealed that the mean values oftotal anaerobic and C.peljringens counts were 9.97x1o3 ± 2.58and 5.13x103 ± 1.08 cfu /g) for Cocktail, 4.12x1o3 ± 0.89 and 1.68x1o3 ± 0.41 (cfu /g). for Salami and 1.55x1o3 ± 0.38 and 6.05x102 ± 1.27 (cfu /g)for Pepperoni, respectively . Anaero­ bic spore forming Clostridial spp. which isolated in different percentages from the ex­ amined samples,included C.peifringens, C.absonum, C.bifermentans, C.butyricum, C.histolyticum, C.pasteurinum, C.putrefaciens, C.sordelli, C.sporogenes. Moreover, Le­cithinase activity ofC.perjringens isolatedfrom the examined samples ofvacuum pack­aged meat products indicated that 5 samples out of 9 Cocktail samples contaminated with C.peljringens were lecithinase +ve while the remaining 4 samples were lecithi­nase +ve. while, 4 Salami samples contaminated with C.peljringens were lecithinase +ve and one sample was lecithinase -ve and 2 samples out of 4 Pepperoni samples contaminated with C.peljringens were lecithinase +ve while the remaining 2 samples were lecithinase -ve.Typiri.g of lecithinase +ve of C.peifringens isolated from the exam­ ined samples of vacuum packaged meat products by intradermal inoculation test of Guinea pig was shown type A,C and Din Coclctail,type A,B and C were detected in Sa­ lami and type A and D were detected in Pepperoni with diffpercentages. The public health importance of the isolated organisms were discussed

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