Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

Abd EL-Dayem, Ghada; Saleh, Gehan; Abd EL-  Elwahab, Reham Abd EL- Raouf

doi:10.21608/mvmj.2021.66416.1040

Impact of dietary Spirulina (arthrospira) platensis on growth performance, gene expression and antioxidant status of quail challenged with salmonella enteritidis.

Document Type : Original Articles

Authors

¹ poultry Diseases Department, animal health research institute, (Mansoura branch),Agriculture Research Center, P.O.Box 246 Dokki,12618-Giza, EGYPT

² Biochemistry, Nutritional Deficiency DEPARTMENT, Animal Health Research Institute(AHRI) (Mansoura branch) Agriculture Research Center (ARC), P.O. Box 246 Dokki, 12618 – Giza, Egypt.

10.21608/mvmj.2021.66416.1040

Abstract

Abstract
Objective: To investigate the effect of dietary Spirulina platensis supplementation on growth performance, organ colonization, expression of inflammatory-related genes, and antioxidant status in quail challenged with Salmonella enteritidis.
Design: Randomized controlled experimental study.
Animals: hundred eighty-seven-day-old Japanese quail.
Procedures: birds were randomly allocated into 6 equal groups. G1, G2, and G3: non challenged and fed abasal diet supplemented with Spirulina at 0, 1, and 2 g/kg diet, respectively. G4, G5, and G6: challenged and fed a basal diet supplemented with Spirulina at 0, 1, and 2 g/kg diet, respectively. At 21 days of age, all challenged groups were orally inoculated with 1ml of (1.00x107 Salmonella enteritidis/ml). The collected samples were serum for determining biochemical and antioxidant parameters, cecal tissue samples for determination of gene expression of inflammatory-related genes, and tissue samples from liver, heart, spleen, and caecum for bacterial reisolation.
Results: The dietary supplementation of Spirulina significantly improved growth performance parameters and reduced organ colonization. The cecal pro-inflammatory gene expressions (IL-6, IL1 ß and TNFα) were significantly downregulated while the anti-inflammatory cytokine (IL-10) was significantly increased. In addition, gene expression of cecal serum amyloid (SAA) was significantly down-regulated. The antioxidant and serum biochemical parameters were improved.
Conclusion and clinical relevance: dietary supplementation of Spirulina could be a helpful strategy for mitigating the harmful effects of Salmonella enteritidis in quail.

Keywords