Histophilus somni (Haemophilus somnus ) is as obligate inhabitant of the mucosal surfaces of bovine and sheep and an opportunisitic pathogen responsible for respiratory disease meningoencephalitis myocarditis arthritis and other systemic infections Al - thought exopolysaccharides (EPS) are a large component of bacterial biofilms their contribution to biofilm structure and function has been examined for only a few organisms here we undertook formation we conducted a transposon random gene knockout to study the generated of H.somni 2336 using the EZ:TN tm >KAN-2< TNP transposome tm these mutants were confirmed by colony blot and PCR of kanamycin resistance gene 25 mutants were confirmed to make less biofilm than the wild type strain ( 30-80% decrease) by creation of mutations in the gene encoding for a homolog of filamentous haemagglutinin (FHA) predicted to be involved in attachment in biofilm process we concluded that this gene might also play a role in EPS production and that EPS might be required for celluar attachment to abiotic surfaces .
Mohamed, E. H. (2009). Crucial role of histophilus somni exopolysaccaride in biofilm foration. Mansoura Veterinary Medical Journal, 11(2), 185-197. doi: 10.21608/mvmj.2009.160927
MLA
El- Hadidy Mohamed. "Crucial role of histophilus somni exopolysaccaride in biofilm foration". Mansoura Veterinary Medical Journal, 11, 2, 2009, 185-197. doi: 10.21608/mvmj.2009.160927
HARVARD
Mohamed, E. H. (2009). 'Crucial role of histophilus somni exopolysaccaride in biofilm foration', Mansoura Veterinary Medical Journal, 11(2), pp. 185-197. doi: 10.21608/mvmj.2009.160927
VANCOUVER
Mohamed, E. H. Crucial role of histophilus somni exopolysaccaride in biofilm foration. Mansoura Veterinary Medical Journal, 2009; 11(2): 185-197. doi: 10.21608/mvmj.2009.160927
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