Finding and Evaluation Modified Embalming fluids in Preservation of Anatomical Specimens

Document Type : Original Articles

Authors

1 Mansoura university faculty of veterinary medecine

2 department of anatomy and embryology Mansoura university

3 department of anatomy and embryolgy Mansoura university

Abstract

Objective: to conflict comparative evaluation of three embalming solutions A, B, C(solution A contains ethanol, citric acid, and glycerin, solution B contains ethanol, sorbitol, and sodium metabisulfite, and solution C contain 10%formalin.)
Animals: 10 male rabbits with an average weight of 1.5-2.5 Kg, and age 2.5 months old
Design: Randomized experimental cadaveric study
Procedures: Ten healthy male rabbits (2 months old with a bodyweight of 1.5–2.5 kg) were divided into two groups; each group contained five rabbits. General anesthesia was induced with the inhalation of chloroform, then the animals were exsanguinated through a cannula inserted into the heart. The embalming fluids were delivered into the body through the left ventricle of the embalmed specimens. Then immersion the cadavers in the same embalming solution and left at +4 ◦C where the animals were examined using several parameters (physical properties and microbial evaluations) after 2 months 2 M, 4 M, 6 M, and one year.
Results: The modified embalming mixtures resulted in cadavers with good dissection properties and a dissection room free of unpleasant smell. The mixtures maintain the haptic and optic properties and, a neglected amount of bacteria or fungi were isolated. We will talk about every mixture individually in the table (1)
Conclusion and clinical relevance: The present study concluded that the modification of the composition of the applied fluid resulted in better preservation of anatomical specimens. This modification is achieved by replacing the glycerin with sorbitol as a modifying agent and citric acid with sodium metabisulphite as an antioxidant.

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