Expression of cathepsin L gene of fasciola gigantica in different developmental stages

Document Type : Original Articles

Abstract

Fascioliasis Is one of the most serious parasiiic infestations of livestock. It is caused by Fasciola hepatica (tremperate regions) and Fasciola gigantica (Tropf.cal reglons),It also has a zoonotic importance, the proteolytic enzyme: cathepsin-L cysteine proteinase (28-28,5 KDa) helps the parasite tn tissue invasion. host lmmune evasion and nutrients intake, This study aimed to check weather Fasciola gigantica flukes depend upon cathepsin-L throughout their life cycle in. snail and mammalian hosts or not, For that cuthepstn-L gene was investigated (n adult and other developmental stages of fasciola glgantica using semi-quantitative RT•PCR assay. Cathepsin-L protein was detected by using sodium dodecyl sulphate- polyacrylamide get electrophoresis [SDS-PAGE) analysts and detection of cathepsfn-L cysteine proteinase antigen in examined stages by Enzyme linked tmmunotransfer blot (EITP) analysts.

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