Rapid immunoperoxidase monolayer assay IPMA for detectio and titration of foot and mouth disease antibodies in sheep in comparison with snt and elisa

doi:10.21608/mvmj.2005.124743

Rapid immunoperoxidase monolayer assay IPMA for detectio and titration of foot and mouth disease antibodies in sheep in comparison with snt and elisa

Document Type : Original Articles

10.21608/mvmj.2005.124743

Abstract

This study describes the evaluation of immunoperoxidase monolayer assay6 (IPMA) for detecting antibodies against serotype O1/93 foot and mou diseas6e virus in sera of infected, vaccinated and random field sera of shee6p. The IPMA results were compared with that obtained by serum neutralization te6st (SNT) and indire6ct enzyme-linke6d immunosobent assay (ELISA). All infect, vaccinated sheep sera tested positive by SNT and ELISA, were posititive by IPMA with amean titers 1.35, 1.77 and 1.83 log10TCID50 one month post experimentally infected sheep or sheep vaccinated with inactivated monovalent gel adjuvavt serotype O1/93 FMDvirus vaccine. 30 out of 50 field sheep sera tested positive by both ELISA and IPMA. Out of the 30 positive sera 27 (90%) revealed neutralizing antibody titers of 0.6 to 1.5 log10 TCID 50. In experminta antibodieslly infected or vaccinated sheep, antibodies against serotype O1/93 could be detected 5 to 7 days following infection or vaccination by ELISA and IPMA. The agreement between IPMA and ELISA was 100% but it was 90%between IPMA and SNT in field samples. The applicability of IPMA as specific andrapid for detection of FMD antibodies was discussed.

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