Comarative studies on attenuated and inactivated oil emulsion egg DROP syndrome (EDS) virus vaccine prepared on chicken liver cell culture and duck eggs vaccine

doi:10.21608/mvmj.2005.124718

Comarative studies on attenuated and inactivated oil emulsion egg DROP syndrome (EDS) virus vaccine prepared on chicken liver cell culture and duck eggs vaccine

Document Type : Original Articles

10.21608/mvmj.2005.124718

Abstract

The EDS-76 vaccine produced either in the allantoic cavity of embryonated duck eggs or in chicken liver cell cultures were comparatively studied as a living attenuated and inactivated oil emulsion vaccines. live attenuated vaccine was prerared by propagation of EDS-76 virus in duck eggs followed by 30 passages on prepared chicken liver (CL) cells. the onset of CPE and best time of virus harvesting was determined for each virus passages on CL cells. 25th passages on CL cells, EDS virus loss its pathogenicity and gave 100% protection to the vaccinated chicks. inactivated virus was prepared in either duck eggs or CL cells. live attenuated and inactivated oil emulsion CL cell adapted EDS vaccines gave high immunity to the susceptible chicks based on lymphocyte blastogenesis assay, serum neutralization test, HI and challenge test as well as the inactivated duck eggs oil emulsion vaccine. the CL cells prepared vaccine gave 100% protection to the susceptible chicken when kept at 4ºc for 4 months.

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