Paste des petils ruminants (PPR) virus as one of Morbillivirus (Paramyxoviridae) is a heat labile virus. In Egypt, as a hot-country-in general we are urgently in need to find out a suitable solvent that may be act as heat tolerant. In this work, we tried to select the best solvent which provide a longer stability of reconstituted PPR vaccine six new solvents were studied in a comparative with the traditional control solvent (physiological saline solution). These solvent soilitions included physiological saline solution, 1 M magnesium sulphate solution (MgSO4). 2.5% lactalbumin hydrolysate solution. a mix-ture of lactalbumin hydrolysate 2.5% and sucrose 5% solution 5% sucrose solution. A mixture of 1 M magnesium sulphate and saline solution. and dimethyl sulfoxide 2.5% solution. By using these solvents solutions. the reconstituting PPR vaccine was kept on ice and at 37C, titration of the virus infectivity on Vero cells was carried out at 0, 1 and 2 hours post reconstitution . it was revealed that the best solvent solution was the mixture of 2.5% lactalbumin hydrolysate and 5% sucrose that could keep the loss in the original virus titer at the most minimum.
(2005). Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine. Mansoura Veterinary Medical Journal, 7(1), 1-8. doi: 10.21608/mvmj.2005.124706
MLA
. "Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine". Mansoura Veterinary Medical Journal, 7, 1, 2005, 1-8. doi: 10.21608/mvmj.2005.124706
HARVARD
(2005). 'Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine', Mansoura Veterinary Medical Journal, 7(1), pp. 1-8. doi: 10.21608/mvmj.2005.124706
VANCOUVER
Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine. Mansoura Veterinary Medical Journal, 2005; 7(1): 1-8. doi: 10.21608/mvmj.2005.124706