The effect of ovarian status and steroidogenic potential of granulosa cells and / or follicular fluid on the maturation of camel oocytes in vitro during rating and non-rating seasons were studied. The camel ovaries were collected immediately after slaughter. The oocytes were aspirated jromfollicles with 8-20 mm diameter for culture in matum tion medium (MM) under dt[ferent co-culture condiH.ons, MM+No GCs (No Granulosa Cells), MM+FGCs (Fresh Gmnulosa Cells}, MM+PGCs {Pre-incubated Granulosa Cells), MM+10%FF (F’ollicu.lar Fluid) and MM+FGCs+ I0%FF. The obtained results revealed that. the distibution of small follicles on ovaries was significantly (P<0.01) higher than the medium and preovulatory fol.licles. The different follicular populations with or without corpora lutea were signtficantly (P<0.01) higher during rating than nonrating sea son. During each season, the numbers of follicles were higher on ovaries w ilh than without corpora lutea. There were no significant dijferences in the numbers of medium and large atretic jiJllicles when two seasons were compared, w hereas the med ium atretic joUicles showed higher significant rate fP<0.01) than large follicles du.ring both seasons. The mean value of grad 1+2 oocy ies was significantly (P<0.01) higher than grad 3+4 oocytes only du.ring rating season, al.though the two groups were higher during rating season. than non-rating one. The highest rates of rnetaphase-JI oocytes were observed after co-culture with. FGCs duririg rating and non-rating seasons, whereas the lowest rates were observed after co-culture with PGCs or FF. Jn all. groups, the rates of metaphase-II oocytes were higher during rating season than non-rating one. The rate of nuclear maturation increased pro- gressively with the dwal.ion of co-culture reaching the highest at 36 hrs during rating secison. During non-rati11g season. the rate of nuclear maturation increased from 12 to 24 hrs and remained cons tant ur1 to 36 hrs.
(2003). Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro. Mansoura Veterinary Medical Journal, 5(1), 11-20. doi: 10.21608/mvmj.2003.122803
MLA
. "Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro". Mansoura Veterinary Medical Journal, 5, 1, 2003, 11-20. doi: 10.21608/mvmj.2003.122803
HARVARD
(2003). 'Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro', Mansoura Veterinary Medical Journal, 5(1), pp. 11-20. doi: 10.21608/mvmj.2003.122803
VANCOUVER
Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro. Mansoura Veterinary Medical Journal, 2003; 5(1): 11-20. doi: 10.21608/mvmj.2003.122803