Study the interaction between ciprofloxacin and ferrous sulphate on biochemical analysis and antibacterial activity in rats

Ciprofloxacin was evaluated as antibacterial agent for albino rats in vitro testing. The antibacterial effect was observed against staphylococcus aurous, Streptococcus pneumonia, E. coli, Bacillus subtitles and Salmonella typhimurium with a minimum in hibitory concentration (MIC) level of 0.28, 0.56, 0.14, 0.56 and 1.12 mg/ml, respectively. On the other hand the antibacterial activity of ciprofloxacin was reduced when combined with ferrous sulphate. Oral administration of rats with ciprofloxacin and ferrous sulphate at doses of 9 mg ciprofloxacin and 1.8 mg ferrous sulphate as elemental iron / 100g body weight, respectively for 7 successive days show significant increase in serum enzymatic activities of AST, ALT and ALK phosphates also level of serum creatinine. The administration of ferrous sulphate with ciprofloxacin reduced serum concentration of ciprofloxacin from 14.5 to 5.4 ug/ml.