Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production
Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreasedserumtestosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promotedtesticular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-inducedtesticular damage in male rats.
Barghout, B., El-Sayed, S., & El-Said, E. (2020). Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production. Mansoura Veterinary Medical Journal, 21(1), 31-35. doi: 10.21608/mvmj.2020.21.105
MLA
Basma Barghout; Shaimaa El-Sayed; El-Said El-Said. "Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production". Mansoura Veterinary Medical Journal, 21, 1, 2020, 31-35. doi: 10.21608/mvmj.2020.21.105
HARVARD
Barghout, B., El-Sayed, S., El-Said, E. (2020). 'Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production', Mansoura Veterinary Medical Journal, 21(1), pp. 31-35. doi: 10.21608/mvmj.2020.21.105
VANCOUVER
Barghout, B., El-Sayed, S., El-Said, E. Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production. Mansoura Veterinary Medical Journal, 2020; 21(1): 31-35. doi: 10.21608/mvmj.2020.21.105