Mansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210901Effects of magnetic water on growth performance and immunity of Nile Tilapia9710419066810.21608/mvmj.2021.88819.1071ENNevien KamelAbdelkhalekMANSOURA - 60 ELGOMHORIA ST, MANSOURA UNIVERSITY, FACULTY OF VETERINARY MEDICINE - 35516 MANSOURA EG
2 Abotaleb street, Gedela, Aldakahlia, Mansoura, Egypt0000-0003-1396-0273AhmedAbdelhamidFish production Department, Faculty of Agriculture, Al-Azhar University, Cairo 11823, Egypt.ShaymaaRezkDepartment of Cytology and Histology, Faculty of veterinary medicine, Mansoura UniversityMohamedMabroukFish production Department, Faculty of Agriculture, Al-Azhar University, Cairo 11823, Egypt.Journal Article20210802<strong><em>Objective:</em></strong> The present study was designed to investigate the effect of magnetic water treatment technique and different feeding rates on growth performance, feed utilization, water quality parameters, chemical composition and intestinal histomorphometric parameters of monosex Nile tilapia (<em>Oreochromis niloticus</em>).<br /> <strong><em>Design:</em></strong> Experimental study with a factorial design.<br /> <strong><em>Fish</em></strong><em>:</em> A total of 2880 apparently healthy monosex Nile tilapia (<em>O. niloticus</em>) with an average initial body weight of 69.86 ± 0.8 g were randomly distributed into 18 concreate ponds.<br /> <strong><em>Procedures</em></strong><em>:</em> Experimental fish were fed on commercial diet (30.1% protein and 4600 Kcal GE/kg diet) based on three levels of feeding rate 3, 4 and 5% from fish biomass for both treated and control groups. Water was treated with magnetic waves at 0.2 Tesla (Tesla= 2000 Gauss) compared to the control group water (zero Tesla). Growth performance parameters, feed utilization, chemical composition and intestinal morphometric analysis were calculated in all groups at the end of the experiment after eight weeks<strong>.</strong><br /> <strong><em>Results</em></strong><em>:</em> The results indicated that growth performance, feed utilization and intestinal histomorphometric analyses improved significantly (<em>P</em><0.01) in magnetic water groups compared to control groups at the three levels of feeding rate. In addition, water physicochemical parameters including Ammonia (NH<sub>4</sub>), Nitrate (NO<sub>3</sub>), Nitrite (NO<sub>2</sub>)<sub>, </sub>PH and dissolved oxygen (DO) significantly improved in magnetic water treated groups at the three feeding rate levels.<br /> <strong><em>Conclusions and clinical relevance:</em></strong> In conclusion, magnetic treatment of water could improve water quality parameters, fish growth performance, feed utilization, and intestinal histomorphometric analyses at different feeding rate.https://mvmj.journals.ekb.eg/article_190668_fd04b6195897e7db7ddffe6610933aad.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210901Adverse effects of moxifloxacin and flunixin meglumine and their combination on pregnant rats10511019393310.21608/mvmj.2021.193933ENEmadAbdAl-SalamDepartment of Pharmacology, Faculty of Veterinary Medicine, Mansoura University 35516 Mansoura, EgyptRashaAbdel-GelilDepartment of Pharmacology, Faculty of Veterinary Medicine, Mansoura University 35516 Mansoura, EgyptMagdyAmerJournal Article20200816<strong><em>Objective:</em></strong> To detail an easy approach to identify the changes that moxifloxacin and flunixin meglumine have caused in the dam of female rats.<br /> <strong><em>Design:</em></strong> Randomized controlled experimental study.<br /> <strong><em>Animals:</em></strong> This study was conducted on thirty-five female (170-200 gm) mature white rats (170-200 gm) and clinically intact, 20 mature male albino rats for the purpose of mating.<br /> <strong><em>Procedures:</em></strong>Once mating has occurred, this is considered the first day of pregnancy. Rats were randomly divided into seven groups 1) a control group 2) The moxifloxacin group 6 days 3) the moxifloxacin group 13 days 4) the flunixin meglumine group 6 days 5) the flunixin meglumine group 13 days 6) the moxifloxacin and flunixin meglumine mg group 6 days 7) the moxifloxacin and flunixin meglumine mg group on day 13. Rats were killed on the days specified for the sixth day and the thirteenth day. Blood and liver samples were collected for biochemical analysis of blood and tissue for pathological examination.<br /> <strong><em>Results:</em></strong> Moxifloxacin and flunixin meglumine and their combination have been shown to have some negative effects in mothers of rats, so we recommend that caution be exercised when using moxifloxacin and flunixin meglumine during pregnancy. It was found that the tested drugs had caused a significant decrease in superoxide dismutase and glutathione levels compared to the control group. The histopathological examination of mothers, who were given moxifloxacin and flunixin meglumine and their mixtures, showed variable alterations in kidney, liver and placenta.<br /> <strong><em>Conclusion and clinical relevance:</em></strong> From the above it is clear that moxifloxacin and flunixin meglumine and their combination have caused some negative effects for pregnant rats, so we recommend that you use caution when using moxifloxacin and flunixin meglumine during pregnancy.https://mvmj.journals.ekb.eg/article_193933_7ff4eab969b67721cb2ff44971d737ca.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210901Applications of titanium dioxide nanoparticles in nanomedicine11111619603610.21608/mvmj.2021.196036ENBasmaAhmedDepartment of Biochemistry and chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptEl-SaidEl-SherbiniDepartment of Biochemistry and chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptGehadEl-sayedDepartment of Biochemistry and chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptMohamedEladlDepartment of Biochemistry and chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptAkiyoshiAkiyoshi TaniguchiNational Institute for Materials Science, JapanJournal Article20200929Nanotechnology is an exciting field that has a profitable outcome due to the increased applications of nanomaterials in many areas such as industry, agriculture, business, medicine and public health. Nanomedicine is a science that studies the application of nanotechnology in medicine. For diseases diagnosis, treatment, monitoring, control and prevention. Titanium dioxide nanoparticles (TiO2 NPs) possess the potential to be used in nanomedicine for cancer diagnosis and therapy due to their advantageous characteristics such as biocompatibility, photocatalytic activity and good optical and electronic properties. This review aims to introduce nanomedicine and nanomaterials and focus on the recent research interest toward TiO2 NPs in medicine.<br /> <strong> </strong>https://mvmj.journals.ekb.eg/article_196036_619b1ab0d0bfdb94d17dc70bc8fb161d.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210930Evaluation of aqueous Ozone as a method to combat multidrug-resistant Staphylococcus aureus tainting cattle meat sold in Wasit marketplaces11712319698810.21608/mvmj.2021.196988ENManalKanaanAgriculture Department/ Technical Institute of Suwaria, Middle Technical University, Baghdad, IraqSuraAbdullahAl- Suwaria General Hospital / Wassit Health Office, Wassit, Iraq.Journal Article20200916<strong><em>Objective:</em></strong>To evaluate the effectiveness of ozonation treatment (1/2 ppm for up to 30 min at 3-7 centigrade) on the persistence of multidrug-resistance Staphylococcus aureus (MDR S. aureus) tainting cattle meat.<br /> <strong><em>Design:</em></strong> Descriptive study<br /> <strong>Procedure: </strong>Nine testers of cattle's meat including imported (n = 3) and locally slaughtered (n = 6), which were stored at -18 centigrade and accepted positive for MDR S. aureus were subjected to aqueous ozone (O<sub>3</sub>) as 1/2 ppm for 15 and 30 min.<br /> <strong><em>Results: </em></strong>The results presented that after ozonation treatment (1/2 ppm at 3-7 centigrade), the whole testers which free from MDR S. aureus were 22.2% & 55.6% for 15 & 30 min, respectively. Additionally, the antimicrobial effectiveness of aqueous O<sub>3</sub> as 1/2 ppm on the decline of MDR S. aureus level (log<sub>10</sub>CFU/ml) was measured against three contaminated testers of cattle's meat. The outcomes publicized that afterward handlings, the total decline of bacterial counts was 2-3 log<sub>10</sub> (CFU/ml) after 30 min at 3-7 centigrade, this decline is extremely noteworthy from the opinion of public health. The effectiveness of aqueous O<sub>3</sub> (0.5 ppm/3-7 centigrade) with carcass drip to MDR S. aureus was assessed and the results displayed that 77.8% and 100% of the testers of aqueous O<sub>3</sub> were negative after ozonation treatments for 15 and 30 min, respectively.<br /> <strong><em>Conclusion: </em></strong>O<sub>3</sub> as 1/2 ppm is exceedingly active in dropping the amount of MDR S. aureus contaminated testers and this decline augmented as prolonged experience time to ozonation treatment. On the other hand, O<sub>3</sub> was highly effective in eradicating MDR S. aureus even in the presence of high levels of organic materials. These outcomes designated O<sub>3</sub> as a substitute promising approach to decline meat contagion with foodborne microorganisms for instance MDR S. aureus.https://mvmj.journals.ekb.eg/article_196988_5a8e714824e801ebabfb6bf12c228179.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210901The Impact of Different Estrus Synchronization Programs on Postpartum Holstein Dairy Cow Reproductive Performance12413019722110.21608/mvmj.2021.93141.1078ENAdelHussienMansoura University, Faculty of veterinary MedicineHebaSharawyMansoura University, Faculty of veterinary MedicineYasserLenisDepartamento de Ciencia Animal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira, ColombiaDanielaJamesFaculty of Agricultural Sciences, University of Applied and Environmental Sciences U.D.C.A. Bogota, ColombiaOzgeTurnaDepartment of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul-Cerrahpasa University, Istanbul, TurkeyEngyRishaDepartment of Clinical pathology, Faculty of veterinary medicine, Mansoura University, Mansoura 35516, EgyptWaelEldomanySamyZaabelDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura, University, EgyptMohammedElmetwallyMansoura University, Faculty of veterinary Medicine0000-0001-7486-7756Journal Article20210926<strong><em>Objective:</em></strong> The purpose of this study was to see how the Ovsynch, modified Ovsynch, presynch, and modified presynch protocols affected postpartum reproductive performance in dairy cows.<br /> <strong><em>Design:</em></strong> Randomized controlled experimental study.<br /> <strong><em>Animals:</em></strong> The current research involved 412 dairy cows.<br /> <strong><em>Procedures:</em></strong> The cows were split into four groups: GnRH was given on day 0, PGF2∝ on day 7, and GnRH on day 9. Group 1: (Ovsynch protocol, n= 117) received GnRH on day 0 and PGF2∝ on day 7. Group 2 (modified Ovsynch, n=113): PMSG on day 0, PGF2 on day 7, and PMSG on day 9. Group 3 (presynch protocol, n=98) got two doses of PGF2 14 days apart, which allowed for a 12-day delay in the start of Ovsynch. Group 4 (modified presynch protocol, n=84) got two PGF2∝ doses 14 days apart, which was 12 days before the start of modified Ovsynch. All of the animals were artificially inseminated 16 hours following their last GnRH or PMSG dose. The ovarian rebound, number of services per conception, days open, and calving interval were the measures for determining reproductive performance for the dairy cows.<br /> <strong><em>Results:</em></strong> The main effect of the synchronisation program showed no significance for ovarian rebound (P >0.05). The S/C, days open and calving intervals were decreased significantly (P<0.05) in the 3rd and 4th groups when compared with the cows that received either GPG or PMSG treatment only. All four groups had a conception rate of 35.04 (41/117), 40.7 (46/113), 44.8 (44/98), and 57.14 (48/84) %, respectively (P<0.05).<br /> <strong><em>Conclusion and clinical relevance:</em></strong> It was investigated that giving two PGF2 injections before Ovsynch, either utilizing GnRH or PMSG methods, improved the reproductive performance of dairy cows. Furthermore, cows given PMSG had a greater conception rate than cows given GnRH.https://mvmj.journals.ekb.eg/article_197221_8e3b9ee3b2508b906bcc232895fd39d0.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320211016Notice of correction: The Effect of Boiling on Stability of Oxytetracycline and Sulfamethazine Residues in Raw Milk using HPLC Method13113119984810.21608/mvmj.2021.199848ENJournal Article20211016At the request of the Journal Editor, the following article has been corrected. Fathy<sup>1</sup> H., El-Toukhy<sup>2</sup> M.E., Sabery<sup>1 </sup>M. El-Sherbiny<sup>2 </sup>M; The Effect of Boiling on Stability of Oxytetracycline and Sulfamethazine Residues in Raw Milk using HPLC Method; Mansoura Veterinary Medical journal 20;2:21-26; DOI: 10.21608/MVMJ.2019.22.105<br /> The name and affiliation of Sabery<sup>1 </sup>M. has been corrected. The list of authors and their affiliation after correction are as follow:<br /> Authors: Fathy<sup>1</sup> H., El-Toukhy<sup>2</sup> M.E., Abd Elhafeez<sup>1</sup> M.S., El-Sherbiny<sup>2 </sup>M.<br /> <sup>1</sup>Animal Health Research Institute, Department of Biochemistry, Toxicology and Feed Deficiency corrected to be Agriculture Research Center, Animal Health Research Institute, Department of Biochemistry, Toxicology and Feed Deficiency, Egypt.<br /> <sup>2</sup>Mansoura University, Department of Food Control and Hygienehttps://mvmj.journals.ekb.eg/article_199848_7b3b3e3b158186ac64bc0c98d2b7996e.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210901Incidence of Yersinia enterocolitica in raw farm and market milk sample13213520302610.21608/mvmj.2021.69799.1049ENMarwa IbrahimEltoukhyFood Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Egypt0000000266205629Mohammed El-SherbiniEl-SayedDepartment of Food Hygiene, Faculty of Veterinary Medicine , Mansoura University, Mansoura 35516, EgyptAisha SaadShoeibFood Hygiene Department, Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20210328<strong><em>Objectives:</em></strong> This study was applied to investigate the incidence of <em>Yersinia</em> enterocolitica in raw farm and market milk and studying the quality drawbacks which might happen in milk as a result of its existence.
<strong><em>Design:</em></strong> Observation study.
<strong><em>Samples:</em></strong>Onehundred raw milk samples.
<strong><em>Procedures:</em></strong> Fifty bulk tank milk <strong>(BTM)</strong>, 25 individual milk & 25 market milk samples were collected from various dairy farms and supermarkets in Dakahlia Governorate, Egypt. The quality assessment of the samples began with evaluating the milk physical characteristics including color, odor, pH and the titratable acidity that reflected the hygienic quality of milk production on farm levels. Then the samples were directed for biochemical and microbiological examination with subsequent PCR approach.
<em>Results:</em> The range of titratable acidity started from 0.11 – 0.20 % lactic acid with a mean value of 0.15 ± 0.004. While for microbiological examination of the samples by using the conventional methods of cultivation on <strong>(CIN)</strong> agar medium, 33 presumptive <em>Yersinia</em> isolates were recovered and intended for biochemical tests among which 5 out of these latter isolates were confirmed as <em>Y. enterocolitica</em> by applying PCR, through detecting the presence of 16srRNA gene. Also, this study evaluates not only the public health of consumers concerning the titratable acidity of the examined samples, but also the economic importance of these retailed samples in supermarkets; as <em>Y. enterocolitica</em> could be transmitted through the consumption of raw milk. <strong> </strong>
<strong><em>Conclusion and Clinical Relevance:</em></strong>The <em>Y. enterocolitica</em> incidence was 29% in the examined raw farm and market milk samples.https://mvmj.journals.ekb.eg/article_203026_b7907bbcc3783db1de3ab30ee0e75521.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721922320210930Comparing in vitro maturation rates in buffalo and cattle oocytes and evaluating the effect of cAMP modulators on maturation and subsequent developmental competence13614020476710.21608/mvmj.2021.204767ENAbdelrahmanAbdulkarimTheriogenology Department, Faculty of Veterinary Medicine, Mansoura University, EgyptAhmedBalboulaTheriogenology Department, Faculty of Veterinary Medicine, Mansoura University, EgyptMagdyBadrArtificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, Giza, EgyptWaelBedirTheriogenology Department, Faculty of Veterinary Medicine, Mansoura University, EgyptMohammedElmetwally0000-0001-7486-7756SamyZaabelTheriogenology Department, Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20210816<em><strong>Objective:</strong></em><em> The purpose of this research was to compare the kinetics and meiotic advancement of cattle and buffalo oocytes, as well as to see how cAMP modulators affected the meiotic progression status of cattle and buffalo oocytes during the oocyte collection process.</em><br /> <em><strong>Design: </strong></em><em>comparing maturation stages times in buffalo and cattle oocytes. Cattle and buffalo oocytes were collected, separated into two groups (standard IVM and extended IVM), and cultivated for five hours in 5% CO2 at 39°C. The sample times for extended IVM are 8, 15, 18, 22, 24, and 30 hours. The nuclear status of each oocyte was assessed to determine how far it had matured at each time sample. Then after, study the effect of cAMP modulators on maturation rates of cattle and buffalo oocytes.</em><br /> <em><strong>Procedures: </strong></em><em>Standard IVM samples were taken at different maturation times, commencing at 8 h and ending at 24 h, while extended IVM samples were taken at 30 h. COCs were placed in a 15-mL sterile centrifuge tube with a warmed 3 percent sodium citrate solution and vortexed at maximum speed for 4 to 8 minutes as needed to remove all cumulus cells before being placed in a warm water bath at 39°C for 5 minutes. After that, the oocytes were mounted on a slide and placed in Coplin jars with a 3:1 methanol/acetic acid solution.</em><br /> <em><strong>Results: </strong></em><em>At any stage of sampling, the percentage of oocytes arrested at the GV stage did not differ significantly between cattle and buffalo oocytes. Furthermore, there was no significant difference between cattle and buffalo oocytes in terms of the percentage of oocytes that reached the MI stage. Moreover,</em><em> t<em>he percentage of oocytes arrested at the GV stage did not differ substantially between cattle and buffalo oocytes when maturation was extended using cAMP modulators at all stages of sampling. </em></em><br /> <em><strong>Conclusion and clinical relevance:</strong></em> <em>Modulating cAMP during oocyte maturation can change oocyte kinetics and increase developmental competence by boosting fertilization, cleavage, and morula rates. Furthermore, there is no significant differences in maturation rates between buffalo and cattle oocytes.</em>https://mvmj.journals.ekb.eg/article_204767_4d92f3573493072b4d445add76ed8663.pdf