Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine
1
8
124706
10.21608/mvmj.2005.124706
EN
Journal Article
2005
01
22
Paste des petils ruminants (PPR) virus as one of Morbillivirus (Paramyxoviridae) is a heat labile virus. In Egypt, as a hot-country-in general we are urgently in need to find out a suitable solvent that may be act as heat tolerant. In this work, we tried to select the best solvent which provide a longer stability of reconstituted PPR vaccine six new solvents were studied in a comparative with the traditional control solvent (physiological saline solution). These solvent soilitions included physiological saline solution, 1 M magnesium sulphate solution (MgSO4). 2.5% lactalbumin hydrolysate solution. a mix-ture of lactalbumin hydrolysate 2.5% and sucrose 5% solution 5% sucrose solution. A mixture of 1 M magnesium sulphate and saline solution. and dimethyl sulfoxide 2.5% solution. By using these solvents solutions. the reconstituting PPR vaccine was kept on ice and at 37C, titration of the virus infectivity on Vero cells was carried out at 0, 1 and 2 hours post reconstitution . it was revealed that the best solvent solution was the mixture of 2.5% lactalbumin hydrolysate and 5% sucrose that could keep the loss in the original virus titer at the most minimum.
https://mvmj.journals.ekb.eg/article_124706_3b4967e55ca9cce729e88e801b8c45c2.pdf
Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
The potential use of guinea pigs and mice as an alternative to sheep and goats for safety testing of peste des petits ruminants live vaccine
73
85
124708
10.21608/mvmj.2005.124708
EN
Journal Article
2005
11
22
Three sterile, potent and identified separate batches of the locally manufactured live pastes des petits ruminants virus (PPRV) vaccine were subjected to safety testing in rodents (Guinea pigs and mice) as well as in small ruminants (sheep and goats). For each vaccine batch, three susceptible animals of each of sheep and goats. Including one pregnant animal per species were inoculated subcutaneously. Each with 1 ml of the vaccine containing 5 log10 TCID50 of the reconstituted randomly selected. Statistically representative samples per batch. Same number & status of animals were held as contact control, inoculated S/C, each with the same volume of normal physiological saline solution as a placebo. Corresponding tests in rodents were done using 10 young and 6 pregnant mice for each of the three vaccine batches. Five young and 3 pregnant Guinea pigs received an intramuscular dose of 0.5 ml of 6log 10 TCID50/ ml per head/ batch. The same dose was given intraperitoneally per head of the rest half number of animals . ten unweaned and 6 pregnant mice recived an intraperitoneal dose of 0.1 ml of 6log10 TCID50/ ml perhead per batch. A similar number of control rodents were given the same dosing volume of normal physiological saline solution per corresponding routes of inoculations, as aplacebo. All tested small ruminants as well as rodents remained absolutely healthy throughout a three weeks observation post inoculations. Pregnant animals gave birth to normal healthy suckling off springs. Non lactating rodents. Sacrificed for post-mortem examinations, were absolutely negative to gross pathological findings. Results obtained would be considered a convincing evidence encouraging the orientation to test the locally produced PPRV vaccine safety in rodents as an alternative to sheep and goats. This alternation might save a lot of expenses, time and effort spent in performing one criterion of the quality control integration system.
https://mvmj.journals.ekb.eg/article_124708_830faf6fef8b5bcb090ff0c1559111e8.pdf
Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
Trills for fungal decontamination on sheep carcasses
87
112
124713
10.21608/mvmj.2005.124713
EN
Journal Article
2005
11
22
The fungal contamination onto the outside (subcutaneous) surface of 20 sheep carcasses, slaughtered and dressed at Mansoura municipal (old-fashioned) abattoir, was analyzed before and after 5 different decontamination trials (two at the abattoir and three at the laboratory). At abattoir, this contamination was surveyed over the round. Flank, shoulder, and neck surfaces each of 10 carcasses before and after abattoir decontamination trials, whereas, at laboratory the same mycological analysis was carried out on the surface of abdominal flap, freshly excised from every of other 10 sheep carcasses before and after laboratory decontamination trials. The presence of yeast and mould contamination was in all triple-swab samples taken from the examined surfaces before decontamination trials (100%). After application of the first abattoir trial, the presence every of yeast and mould contamination was recognized in 80 – 100% of round, flank, shoulder, and neck samples of carcass surfaces hose-sprayed with tap water for one minute, while the second abattoir decontamination trial could decrease the yeast-contaminated samples to 50 – 80 %, and mould – contaminated samples to 70 – 90% over the same carcass surfaces hose-sprayed with tap water for one minute then wiped with sterilized cloth unti removal most of visible dirts. Yeast and mould contamination were also detected in 40 and 50 % samples of abdominal flap surfaces sprayed with 0.27% benzoic acid solution for one minute (first laboratory decontamination trial). In 50 and 80% samples of abdominal flap surfaces sprayed with 2% acetic acid solution for one minute (second laboratory decontamination trial), and in 80% samples (each) of abdominal flap surfaces sprayed with 2.5% potassium sorbate solution for one minute (third laboratory decontamination trial), respectively.
https://mvmj.journals.ekb.eg/article_124713_a64dd3d5fc478ef4a5ed71fe7124df43.pdf
Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
Comarative studies on attenuated and inactivated oil emulsion egg DROP syndrome (EDS) virus vaccine prepared on chicken liver cell culture and duck eggs vaccine
113
124
124718
10.21608/mvmj.2005.124718
EN
Journal Article
2005
11
22
The EDS-76 vaccine produced either in the allantoic cavity of embryonated duck eggs or in chicken liver cell cultures were comparatively studied as a living attenuated and inactivated oil emulsion vaccines. live attenuated vaccine was prerared by propagation of EDS-76 virus in duck eggs followed by 30 passages on prepared chicken liver (CL) cells. the onset of CPE and best time of virus harvesting was determined for each virus passages on CL cells. 25th passages on CL cells, EDS virus loss its pathogenicity and gave 100% protection to the vaccinated chicks. inactivated virus was prepared in either duck eggs or CL cells. live attenuated and inactivated oil emulsion CL cell adapted EDS vaccines gave high immunity to the susceptible chicks based on lymphocyte blastogenesis assay, serum neutralization test, HI and challenge test as well as the inactivated duck eggs oil emulsion vaccine. the CL cells prepared vaccine gave 100% protection to the susceptible chicken when kept at 4ºc for 4 months.
https://mvmj.journals.ekb.eg/article_124718_40a55dfbe3fb7b221e2b87096b87f7dc.pdf
Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
Comparative evaluation of the homogeneity and heterogeneity between rabies and bovine ephemeral fever hyper immune sera when used serologically
125
135
124721
10.21608/mvmj.2003.124721
EN
Journal Article
2005
11
22
Hyper immune sera were prepared in rabbits and cattle against rabies and bovine ephemeral fever (BEF) viruses with the conjugation of some portions of them with FITC. Neutralizing antibodies were estimated in each serum and it was found that rabies antibody titers were higher in rabbits than in cattle while those of BEF were higher in cattle than in rabbits. Homologous and heterologous application of SNT, AGPT and direct fat revealed that the homologous tests resulted in higher titers and more clear reactions than in hetreogenous tests indicating that there is a little antigenic relationship between rabies and BEF viruses. This relation may be used in the diagnosis of any of the two diseases in the absence of its specific antiserum but with taking in consideration the case history of animal biting and history outbreaks of BEF in addition to clinical symptoms.
https://mvmj.journals.ekb.eg/article_124721_380865618aa6349e54181c72ee8324af.pdf
Mansoura University, Faculty of Veterinary Medicine
Mansoura Veterinary Medical Journal
1110-7219
7
1
2005
06
01
Post mortem DNA analysis for determining time of death and sex identification
149
166
124739
10.21608/mvmj.2005.124739
EN
Journal Article
2005
11
22
Time if death is usually estimated by evaluating events mostly occur after death. In this paper the potential application of nuclear DNA image analysis in liver tissue used to evaluate post mortem interval. After death, internal nucleases within the cells cause degradation of nuclear DNA of hepatocyte more rapidly than other tissue , where liver cell rich in lysosomes that contain hydrolases enzymes. Nuclear DNA degradation is measurable and quantifiable in relation to time. Three liver samples were taken at zero, 24, 48, 72 hours, 5 days, one and two weeks post mortem interval. DNA ploidy, DNA index (1&2), mean DNA content, nuclear area and DNA histogram were found to correlate with an increased post mortem interval from zero to 72 h. hepatocyte nucleus and sequenced DNA histogram couldn’t be detected at or more than 5 days after death. Three skeletal muscle samples ( at zero, 24, 72, 1w, 2w, 19 days and 21 days)were used for sex identification using polymerase chain reaction (PCR) technique. Sex could be identified till two weeks post mortem interval. from these results nuclear DNA in hepatocyte reveals time dependent alteration. So can used as a predictor for post mortem interval. DNA in certain tissue (Skeletal muscle) is more stable and allowing to perform DNA typing (sex identification) by polymerase chain reaction.
https://mvmj.journals.ekb.eg/article_124739_5edb4e620d0b84351795845943c078b0.pdf