Mansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171218A SURVEY ON BACTERIAL DISEASES CAUSING MORTALITIES IN THE FIRST WEEK OF BROILER,S CHICKS LIFE27328811834110.21608/mvmj.2017.118341ENJournal Article20170904<span>The present study was conducted to investigate bacterial diseases causing mortality in the first week of broiler’s chick life on broiler poultry farms in Dakahlia Governorate. A total of 240 samples (105moribund chicks and 135 dead chicks) resembles different breeds (Avian,Cobb, Hubbard, Baladi and Saso) , different ages(1-7d.), different housing capacity (5.000:20.000) as well as different farm districts in Dakahlia Governorate were collected during the experiment period (2012-2015). The isolated bacteria from these samples were identified by culture CHARacter , biochemical tests and serological identification. The predominant isolated bacteria were Salmonella (75%) identified as 3 isolates of Salmonella Enteritidis one isolate of Salmonella Typhimurium , one isolate of Salmonella Kentucky one isolate of Salmonella Tamale and E. coli isolates (25%) identified as two isolate of E. coli (O)serogrroup. The isolated strains of Salmonella and E. coli were inoculated into susceptible one day old chicks by two routes (oral and I/p) route. The clinical signs, PM lesions, histopathological lesions and performance profile of each subgroup revealed that chick mortality were recorded with clinical signs and septicemic pictures of most inoculated groups except control group. Mortality rate was higher in (I/p) inoculation than (oral) inoculation . Histopathological lesions proved signs of severe congestion in liver, lung, and other tissue samples. Growth performance parameters proved that there were significantly lower feed intakes, mean body weight gain all over the experimentation period (four weeks) in all groups in comparison with control group.</span>https://mvmj.journals.ekb.eg/article_118341_35c24d422de0b266c1466d6e34b640ec.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120170601PROTECTIVE EFFECTS OF WHEAT GERM OIL AGAINST HYPERLIPIDEMIA INDUCED-ISCHEMIC BRAIN STROKE AND OXIDATIVE STRESS IN HYPERCHOLESTEROLEMIC AND STZ-DIABETIC RAT MODELS31933211834510.21608/mvmj.2017.118345ENJournal Article20201012<span>Thiswork aimed to study protective effects of oral administration of wheat germ oil (WGO 500 mg/kg B.wt) on hyperlipidemia and brain oxidative stress in hypercholesterolemic (HC) and streptozotocin (STZ) -diabetic rats. Five groups of male Sprague Dawely rats were used; group 1: control negative, group 2: HC rats, group 3: STZ- diabetic rats, group 4: HC rats treated with 500mg/kg B.wt WGO and group 5: STZ-diabetic rats treated with 500mg/kg B.wt WGO. After 6 weeks (the end of the experimental period), serum of all rat groups were used for lipid profile analysis to determine the levels of total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein cholesterol (LDL-C), very low density lipoprotein cholesterol (VLDL-C), high density lipoprotein cholesterol (HDL-C), TGs/HDL-C ratio and TC /HDL-C ratio. Brain tissue homogenates from all rat groups, were used for evaluation of malondialdehyde (MDA) level, antioxidant enzymes activities like superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH). Specimens from cerebrum were microscopically examined. HC rats (G2) and STZdiabetic rats (G3) showed a significant elevation of serum lipid profile, atherogenic index (AI) and brain MDA level, while there was a significant decrease in serum HDL-C and antioxidant enzymes activities in brain tissue homogenate when compared with control negative group (G1). Oral administration of 500mg/kg B.wt WGO to HC and STZ-diabetic rats, significantly (p ˂ 0.05) reduced serum lipid profile, AI and brain MDA, however significantly (p ˂ 0.05) increased serum HDL-C levels and antioxidant enzymes activities in brain tissue homogenate when compared with untreated HC and STZ- diabetic rats. Histopathological examination of cerebral tissue revealed that WGO treatment of HC and STZ-diabetic rats showed normal histology. Abbreviations: WGO, wheat germ oil; B.wt, body weight; TC, Total cholesterol; STZ, streptozotocin. TGs, Triglycerides; LDL-C, Low density lipoprotein- cholesterol; VLDL-C, Very low density lipoprotein- cholesterol; HDL-C, High density lipoprotein-cholesterol; AI, Atherogenic Index ; MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH, reduced glutathione; PBS, phosphate buffer saline; H&E, hematoxylin and eosin</span>https://mvmj.journals.ekb.eg/article_118345_7c20bc40425e548ef0dce328ed44d8df.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212DIFFERENTIATION OF HUMAN OLFACTORY BULB NEURAL STEM CELLS INTO OLIGODENDROCYTES19520712568310.21608/mvmj.2017.125683ENZ. AShouman,Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,AAbd-Elmaksoud,Department of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,SLashenDepartment of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt,Hany E.MareiDepartment of Cytology and Histology, Faculty of veterinary medicine, Mansoura University, Egypt, and Biomedical
Research Center, Qatar University, Doha, QatarJournal Article20170815<span>Human olfactory bulb neural stem cells (OBNSCs) are multipotent cells that are capable of differentiation into neurons, astrocytes, and oligodendrocytes. In the present study, we investigated the ability of human OBNSCS to differentiate into oligodendrocytes using RT-PCR and immunocytochemistry. In the presence of fetal calf serum, all-trans retinoic acid (RA), triiodothyronine (T3), and cyclic adenosine monophosphate (cAMP), OBNSCS were differentiated into oligodendrocytes precursors as evidenced by induction and repression of oligodendrocytes chondroitin sulphate proteoglycan (NG2) and nestin genes, respectively. These finding were confirmed at the protein level using fluorescent immunocytochemistry (FICC) according to standard protocol. Taken together, the present study highlighted the ability of OBNSC to differentiate into oligodendrocytes, a finding that indicates their possible use as a promising candidate for cell-based therapy for neurodegenerative and traumatic diseases associated with myelin impairments. Abbreviations: Human olfactory bulb neural stem cells (OBNSCs), retinoic acid (RA), triiodothyronine (T3), cyclic adenosine monophosphate (cAMP), pro-oligodendrocytes chondroitin sulphate proteoglycan (NG2), immunocytochemistry (FICC).</span>https://mvmj.journals.ekb.eg/article_125683_35b22bf69f82aaf78b4727a671d54e8c.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212NEURAL DIFFERENTIATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS18319312584910.21608/mvmj.2017.125849ENA MEl-GamalDepartment of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, EgyptAAbd-ElmaksaudDepartment of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, EgyptAFaragDepartment of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, EgyptH EMareiDepartment of Cytology and Histology Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20170912<span>The limited neurodegenerative power, makes from neural disorders the most challenging and difficult therapy among different diseases. till now, the traditional surgical and medical interventions only slow down the progression of these neurodegenerative diseases but the number of neurons are still diminish in many patients. Now, the mesenchymal stem cell (MSC) therapy has attracted the great attention of most scientists and clinicians around the world to be used as a therapeutic potential source for different neurodegenerative disorders. Stem cells have been found all over the body and can be obtained from different tissues as; adipose tissue, bone marrow and blood. MSCs CHARacterized by high proliferation and differentiation abilities, providing a source of neurons and glia for cell replacement therapy. Also, MSCs show neuroprotective effects without any genetic modification and not show teratoma. Lately, scientist directed toward differentiation of mesenchymal stem cells toward neural cells using different growth factors and chemicals. In the current study, we have tested the effect of ATRA in a dose 10µM and heparin in a dose of 50µg as neural inducers either for dopaminergic or cholinergic neurons. The results indicate that both retinoic acid and heparin are positively neural inducers. Also found that heparin mixed with retinoic acid give better results. These results were confirmed using RT-PCR for MAP2, β-tubulinІІІ, ChAT, TH neural genes markers.</span>https://mvmj.journals.ekb.eg/article_125849_cdd2227e61d644f3c2fbfeff9d27c36f.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PROTECTIVE EFFECT OF SOME RENIN INHIBITORS IN ACUTE NEPHROTOXICITY INDUCED BY SOME ANTICARCINOGENIC DRUGS IN RATS33334612585010.21608/mvmj.2017.125850ENSEL-SherbiniDepartment of Biochemistry and chemistry of nutrition.
Veterinary Medicine, Mansoura UniversityG REL-SayedDepartment of Biochemistry and chemistry of nutrition.
Veterinary Medicine, Mansoura UniversityShimmaaAhmedDepartment of Biochemistry and chemistry of nutrition.
Veterinary Medicine, Mansoura University, Animal Health research instituteJournal Article20170914<span>Investigation was carried out to study protective effect of some renin inhibitors in acute nephrotoxicity on forty male Sprague Dawely rats weighting 200g to 250g which divided into four equal groups each group included 10 rats GroupI (Control group): was fed on normal ration for one month. While GroupII (cisplatin group) was injected i.p. by 7.5 mg kg B.W of cisplatin for one month. GroupIII (captopril group) was injected i.p. by 60 mg kgB.W of captopril for one month.GroupIV (captopril plus cisplatin group) there is injection of cisplatin (7.5mg/kg b .wt) i.p and within one hour it is proceeded by i.p injection of captopril (60 mg/kg b. wt) daily for one month. At the end of experiment the obtained results revealed that, administration of cisplatin and captopril caused a highly significant decrease in serum and kidney MDA ,serum creatinine concentration , serum blood urea nitrogen concentration, serum angiotensin II concentration and serum aldosterone level. whole blood and kidney p53 concentration compared to cisplatin treated rats .Also there was highly significant increase in whole blood GSH concentration, serum Glutathione peroxidase, serum catalase activity, serum superoxide dismutase activity and serum renin concentration compared to cisplatin treated group. Administration of cisplatin only revealed in increase in serum and kidney MDA ,serum creatinine concentration , serum blood urea nitrogen concentration, serum angiotensin II concentration serum aldosterone level, whole blood and kidney p53 concentration as compared to control group . There was a highly significant decrease in whole blood GSH concentration, serum Glutathione peroxidase, serum catalase activity, serum superoxide dismutase activity and serum renin concentration compared with control group. Administration of captopril only result in decrease in serum and kidney MDA ,serum creatinine concentration , serum blood urea nitrogen concentration, serum angiotensin II concentration serum aldosterone concentration, whole blood and kidney p53 concentration compared to control group also there was highly significant increase in whole blood GSH concentration, serum Glutathione peroxidase, serum catalase activity, serum superoxide dismutase activity and serum renin concentration when compared to control group. These results were supported by histopathological examination which revealed some alterations represented by that the tubule was dilated and shrinked or atrophied , the tubular cells was removed and lost and there is asignificant destruction of glomerular with penetration of leuococyte and intratubular bleeding. While administration of cisplatin and captopril result in regenerating glomeruli and tubules of kidney tissues produce by cisplatin administration.</span>https://mvmj.journals.ekb.eg/article_125850_f951c75011fe27c9418b4bab294d78cb.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PATHOLOGICAL DIAGNOSIS OF SKIN BIOPSIES FOR DIFFERENT OUTBREAKS OF CAMEL POX VIRUS INFECTION IN EGYPT AND UAE17518212642010.21608/mvmj.2017.126420ENAbdullahMORSIPathology Department, Faculty of Veterinary Medicine, Zagazig University.
Pathology Department, Faculty of Veterinary Medicine, Mansoura University.
Abu Dhabi Food Control Authority, Al Ain Central Veterinary Hospital.AhmedELNAGAAR*Pathology Department, Faculty of Veterinary Medicine, Zagazig University.
**Pathology Department, Faculty of Veterinary Medicine, Mansoura University. ***Abu Dhabi Food Control Authority, Al Ain Central Veterinary Hospital.MohamedMOUSTAFA*Pathology Department, Faculty of Veterinary Medicine, Zagazig University.
**Pathology Department, Faculty of Veterinary Medicine, Mansoura University. ***Abu Dhabi Food Control Authority, Al Ain Central Veterinary Hospital.Journal Article20170912<span>In this study, skin biopsies of two outbreaks of camel pox infection in camels were collected from Belbeis, Sharkeya governorate, Egypt and Al Ain city, Abu Dhabi, United Arab Emirates (UAE) for histopathological diagnosis and polymerase chain reaction (PCR) CHARacterization and identification. Camel pox virus (CPV) reported as a specific viral disease of camel. CPV is highly contagious mainly in calf camels and usually mild in intensity.The mild form, CHARacterized by papular, pustules on the lips, nostrils, cheeks and different parts of skin. CPV usually associated by lymphadenopathy and nasal discharge . This study declared numerous outbreaks of mild pathogenicity for camel pox virus and the causative agent was identified and confirmed as camel pox virus (CPV) by PCR</span>https://mvmj.journals.ekb.eg/article_126420_670bd68470529b894355e8ea5f4d62f7.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ANTIOXIDANT TRACE ELEMENTS AND OXIDATIVE STRESS STATUS IN DAIRY CATTLE SUFFERING from MASTITIS16517412642510.21608/mvmj.2017.126425ENMedhat NassifNassifDepartment of Animal Medicine, Faculty of Veterinary Medicine, Kafr El-sheikh University, Kafr El-sheikh, EgyptHussam MohamedIbrahimDepartment of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptHeba AdeMoharamDepartment of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptJournal Article20170912<span>Mastitis is an inflammation of the mammary glands caused by several pathogens. Reactive oxygen species are natural end products of the intensive cellular metabolism. Oxidative stress occurs when there is a disturbance of homeostasis causing inflammation of the mammary gland. This study was performed to evaluate blood antioxidant profile and trace elements in dairy cows with clinical mastitis. For this aim, venous blood samples were collected from 116 cows (100 mastitic, 16 control). In cows with clinical mastitis, there was a significant (p < 0.05) increase in the clinical index score compared with control group. Biochemically, there was a significant (p < 0.05) decrease in super oxide dismutase, catalase and total antioxidant capacity as well as in the level of zinc. However, there was a significant (p < 0.05) increase in the levels of malondialdehyde and glutathione reductase as well as in that of copper. The results of the current study indicate that the body antioxidant defense system was compromised in dairy cows with clinical mastitis creating a state of oxidative stress.</span>https://mvmj.journals.ekb.eg/article_126425_a7d74693fa81e8ee2440112e6f41bdf1.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212HEPATO-RENAL ALTERATIONS IN DAIRY CATTLE WITH SUBCLINICAL KETOSIS DURING TRANSITION PERIOD15516312643010.21608/mvmj.2017.126430ENMohamedYoussefDepartment of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine Mansoura University, Mansoura 35516, EgyptMagedEl-AshkerDepartment of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine Mansoura University, Mansoura 35516, EgyptMarwaYounisDepartment of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine Mansoura University, Mansoura 35516, EgyptJournal Article20171112<span>The purpose of the present study was to evaluate hepatic and renal functions in dairy cows suffered from sub clinical ketosis during the transition period. For this purpose, 730 Holstein Friesian dairy cattle, from a commercial dairy farm were included in this investigation. On the farm, only twenty four cows were located at a late gestation period. Blood samples were collected from each of the transition cows to estimate serum levels of β-hydroxy butyric acid (BHBA).Cows with no remarkable clinical signs but having serum BHBA> 1.20 mmol/L to 2.9 mmol/L were considered to have SCK (n = 20), while cows with no clinical signs and serum BHB < 1.00 mmol/L were considered controls (n = 4).Blood samples were then used to estimate serum values of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen , and creatinine, total protein, albumin and globulin. Our findings demonstrated significant higher values of BHBA (P<0.05), blood urea (P = 0.019), AST (P = 0.021), and ALT (P = 0.001) in diseased cows than those in controls; while creatinine, total protein and globulin showed no significant changes. We conclude that cows with SCK demonstrated marked hepatic dysfunction with substantial evidences of fatty liver. Further studies are needed to evaluate the glomerulus filtration rate in the diseased cattle that have normal serum creatinine concentrations and suspected kidney disease.</span>https://mvmj.journals.ekb.eg/article_126430_9003a7dded9195fcfbd81d6528847914.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212CORRELATION BETWEEN ABNORMAL OVARIAN STRUCTURES AND UTERINE HISTOPATHOLOGICAL ALTERATIONS IN SHE-DONKEY14315312643410.21608/mvmj.2017.126434ENKhalidEl-NesrPathology Department, Faculty of Veterinary Medicine, Beni-Suef UniversityWalaaAwadinPathology Department, Faculty of Veterinary Medicine, Mansoura UniversityOmarAbd El-WahedPathology Department, Faculty of Veterinary Medicine, Mansoura UniversityJournal Article20170912<span>In this study, which extended from May 2014 to December 2015, histopathologic techniques made on both ovaries and uteri of 83 slaughtered she-donkeys. Histopathologically, variable ovarian abnormalities were recorded including follicular cyst, paraovarian cyst, granulosa cell tumor, cavernous hemangioma, oophoritis and ovarian hematoma. In correlation to these abnormalities, the uterine examination revealed variable degrees and types of pathological alterations; some of which were relied on these ovarian abnormalities and others were not</span>https://mvmj.journals.ekb.eg/article_126434_991a1e07c43850f9eeb33e0092020a3f.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EVALUATION OF NON GENETIC FACTORS AFFECTING MILK PRODUCTION TRAITS OF HOLSTEIN CATTLE13314212643710.21608/mvmj.2017.126437ENM MFouda,Department of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, EgyptKh MEl- BayomiDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptR ADarwishDepartment of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, EgyptA REltanahyDepartment of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20171112<span>This study was carried out to evaluate the effects of a ge at calving (AC), parity, days in milk (DIM), calving interval (CI), d ays open (DO), d ry period (DP), number of services per conception (NSC), s eason of calving on peak milk yield (PMY), time to attain peak milk yield (TPMY), 305 day mature equivalent (305- DMY) and total milk yield (TMY). Age at calving had significant effect (P≤ 0.05) on all traits studied except TPMY. DIM had a highly significant effect (P< 0.001) on PMY (37.14- 39.18 kg), TPMY (99.29- 194.08days), 305-DMY (8692.86 - 9760.65 kg) and TMY (4552.82- 12416.95 kg) respectively. Furthermore, DO and DP had a highly significant effect on PMY (33.72- 40.29 and 31.81 41.08 kg, respectively), TPMY (152.01- 165.14 and 139-165 day s , respectively), 305-DMY (8613.83- 9606.83 and 7266.76- 9699.76 kg, respectively) and TMY (8057.51- 8865.18 and 6682.97- 8850.30 kg, respectively). NSC had a highly significant effect (P< 0.001) on all traits studied except PMY. On the other hand, parity had no significant influence (P ≥ 0.05) on all traits studied except PMY. CI had no significant effect on all traits studied except 305-DMY. Moreover, season of calving had no significant effect on all traits studied. In conclusion, these factors should be accounted for genetic evaluation process. At farm level, management decisions such as selection and culling, and feeding should made putting these factors into consideration in order to achieve more reliable evaluation.</span>https://mvmj.journals.ekb.eg/article_126437_662685bf4da1cd790c998e07c36eb449.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECT OF FLAXSEED ON SERUM LIPIDS AND ATHEROSCLEROSIS IN RABBITS26327112643910.21608/mvmj.2017.126439ENEl- Said El-SaidEl- SherbiniDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, EgyptGehad REl-sayedDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, EgyptMahmoud GEl-sebaeiDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, EgyptFatmaHassanDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20170912<span>This study was carried out to investigate the beneficial effect of flaxseed-rich diet on atherosclerotic rabbits. Rabbits were divided to 4 groups: group I, control diet, group II,1%cholesterol diet, group III, 10% flaxseed diet, and group IV, 1% cholesterol+ 10% flaxseed diet. Blood and tissue samples were collected after 2 months for measurement of serum lipid profile :total lipids, total cholesterol (TC), triacylglycerol (TAG), HDL-cholesterol (HDL-C), LDL- cholesterol(LDL-C) ;blood and tissue antioxidants :reduced glutathione (GSH) ,superoxide dismutase (SOD) and catalase ;oxidative stress parameters :nitric oxide(NO) ; serum lipid peroxidation product : malondialdehyde (MDA) and histopatholgical examination of aorta. The results of the current work indicated that dietary cholesterol supplementation significally increased lipid profile parameters ;total lipids, total cholesterol(TC), triacylglycerol (TAG), HDLcholesterol (HDL-C) and LDL-cholesterol (LDL-C),MDA and tissue SOD. On the other hand, cholesterol supplementation reduce NO, catalase, blood SOD and blood GSH but there was no change in tissue GSH .Also the result indicated that cholesterol and flaxseed supplementation on the same time lead to increase lipid profile parameters (total lipids, total cholesterol (TC), HDLcholesterol (HDL-C) and LDL-cholesterol (LDL-C) but not affect triacylglycerol (TAG), also, increase MDA, NO, catalase and tissue SOD but not affect blood SOD and decrease blood GSH , but not changed in tissue. On the other hand flaxseed supplementation alone was resulted in increased total lipids ,slight increase in total cholesterol (TC) ,there was no change in triacylglycerol (TAG), decrease HDL-cholesterol (HDL-C) ,increase LDL- cholesterol(LDL-C), no change in MDA and NO, increase blood catalase, liver GSH, but decrease tissue catalase ,blood GSH. from these results it can be concluded that flaxseed assured no protection against atherosclerosis in cholesterol fed rabbits.</span>https://mvmj.journals.ekb.eg/article_126439_41aeb9224463f6c80454d1105c1cf857.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ECONOMIC EFFICIENCY OF CHICKEN BREEDS ON BROILER PRODUCTION11713112660210.21608/mvmj.2017.126602ENMFoudaAnim. Husb. And Anim. Wealth Dev. Dept. Fac. Of Vet. Med. Mansoura Univ.S TAtallahAnim. Husb. And Anim. Wealth Dev. Dept. Fac. Of Vet. Med. Alex. Univ.R ADarweishAnim. Husb. And Anim. Wealth Dev. Dept. Fac. Of Vet. Med. Mansoura Univ.NohaWahedAnim. Husb. And Anim. Wealth Dev. Dept. Fac. Of Vet. Med. Mansoura Univ.Journal Article20170812<span>This study aimed to investigate the difference in productivity between different broiler breeds and their effect on productive and economic efficiency of broiler farms through studying, the effect of most important factors that may affect broiler farm production (season of production, broiler breed and system of housing) and the effect of breed among different seasons and housing systems on total return, total costs and net profit. Also, the effect of veterinary management (Drugs, vaccines, disinfectant, and veterinary supervision), mortality rate, marketing age, marketing weight and marketing price of Kg meat on farm production were evaluated. This study was carried out during the period from 2012 – 2015 on random cycles of broiler farms in four different Provinces including Dakahlia, Kafr Elsheikh, Gharbiya and Sharqia. Our study concluded that, in order to gain high profits from broiler farming, it is important to achieve productive and economic efficiency. Also, It was found that the best economical broiler breeds in the study were Sasso, Ross and Hubbard breeds where the high total return and net profit were obtained. On the other hand, winter season was better than summer season for broiler production, where the farm gave higher total return and net profit. In broiler production the veterinary management inputs (vaccines, drugs, veterinary supervision and disinfectants) were very important and represented about 13% from the total variable cost</span>https://mvmj.journals.ekb.eg/article_126602_30d3971e368a7a62f089b5b38a28f84f.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ISOLATION AND IDENTIFICATION OF INFECTIOUS BURSAL DISEASE VIRUS (IBDV) from INTERNAL ORGANS OF VACCINATED CHICKENS IN DAKAHLIA GOVERNORATE, EGYPT9510412660310.21608/mvmj.2017.126603ENA AEl-KenawyDepartment of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, EgyptA AAminDepartment of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, EgyptMEl-TholothDepartment of Virology, Faculty of Veterinary Medicine Mansoura University, Mansoura, EgyptJournal Article20170912<span>In the present study, a total of 35 samples including bursa of Fabricius, thymus, spleen,kidney and liver (7samples for each organ) were collected from vaccinated chickens flocks at Dakahlia Governorate, Egypt during the period from March 2015 to May 2015. Each sample was pooled randomly from three to five birds (25 to 44 days old). Trials for isolation of the suspected virus from the collected samples were carried out via chorioallantoic membranes (CAMs) of 9 days embryonated chicken eggs (ECEs), collected from hens free from infectious bursal disease virus (IBDV). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard IBDV. IBDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). The results revealed that 30 samples (85%) including 7 (70%) samples from bursa of Fabricius and 6 (60%) samples from thymus, spleen and liver and 5(50%) samples from kidney, showed positive results in virus isolation. The positive results percentage by AGPT and IFAT for field samples were 88.5% (31 out of 35samples), while after 3rd passage in ECEs were 70% (22 out of 35 samples) and 88.5%(31 out of 35samples) respectively. The results indicate that the bursa of Fabricius is the best organ for IBDV isolation and AGPT and IFAT could be used for IBDV diagnosis.</span>https://mvmj.journals.ekb.eg/article_126603_2bcf663d42f75878b7c3e7c888a4f7bb.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECTS OF DIETARY PROTEIN AND THREONINE LEVELS ON GROWTH PERFORMANCE AND IMMUNOCOMPETENCE IN BROILER CHICKENS759312685010.21608/mvmj.2017.126850ENM AHassanDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptO AOrmaDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptT IMohamedDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptA MOrmaDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptJournal Article20170812<span>The present work was conducted to study the effects of feeding low crude protein diets (LCP-diets) with supplementation of threonine (Thr) on growth performance and immunocompetence in broiler chickens. One day old 264 Cobb chicks were divided into 6 equal groups (each of 4 replicates and each replicate was of 11 chicks). The 1st group is considered the control one and fed normal basal control starter, grower and finisher diets containing the recommended levels of CP, threonine, methionine, lysine, tryptophan, valine, arginine and metabolizable energy as specified for Cobb500 broiler performance. The 2nd experimental group was fed ideal protein diets which were formulated to contain the minimum nutrient specifications based upon supplementing digestible essential amino acids (lysine, methionine, tryptophan, threonine, arginine, and valine). The other experimental chick groups (3rd, 4th, 5th and 6th groups) were fed isocaloric diets containing low levels of total dietary CP% (1 and 2% less than the minmum recommended CP%) with supplementation of Thr to 120 or 140% of the minimum recommended levels. The diets were isocaloric (3035, 3108 and 3180 Kcal/kg for starter, grower and finisher diets respectively). All chicks were vaccinated against Newcastle disease virus (NDV) at day 14 of age through eye drops. Growth performance indices (body weight, feed intake, feed conversion ratio and protein efficiency ratio) were measured. Blood samples were collected from the broilers at 14, 21, 28 and 35 days of age to determine the serum titre of antibodies against NDV using haemagglutination inhibition test (HI). At 42 days of age, blood samples were collected for determination of serum metabolites and 12 chickens from each group were slaughtered to obtain some of the carcass traits. The results revealed that BW and FCR of the broilers fed 2% LCP- diets with 120 or 140% Thr supplementation were significantly lowered than those fed control or ideal protein diets, as well as those fed 1% LCP- diets with Thr supplementation. There were no differences between the groups in dressed carcass percentages. The abdominal fat % in the carcass of the broiler chickens was not significantly affected by decreasing the dietary CP or supplementation of threonine. Liver weight% significantly increased with feeding the 2% LCP- diets. Feeding 2% LCP- diets also resulted in significant increase in serum uric acid and decreased serum total protein, albumin and globulin. The highest HI titer values and lowest serum levels of uric acid were observed in chickens fed ideal protein diets. While, chickens fed LCP- diets (-2%) had reduced HI titer values. from the present study, it could be concluded that the dietary protein level could be decreased by 1% of the recommended levels with threonine supplementation to 120 % of requirements with no adverse effects on body weight development, FCR, serum metabolites and immune competence in broiler chickens. Formulating broiler diets based upon digestible essential amino acids achieved optimal growth performance and maintained immunocompetence.</span>https://mvmj.journals.ekb.eg/article_126850_2ae8fcdc0a26635797efc4f3d7423897.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212MOLECULAR DIAGNOSIS OF CASEOUS LYMPHADENITIS IN SHEEP AT DAKAHLIA GOVERNORATE, EGYPT677412685310.21608/mvmj.2017.126853ENA MSelimInternal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura UniversityS MAtwaInternal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura UniversityA AEl-GedawyBacteriology Department ,Animal Health Research Institute ,Dokki, GizaA EYounisInternal medicine , Infectious and Fish diseases Department ,Faculty of Veterinary Medicine ,Mansoura UniversityJournal Article20170912<span>The aim of the present study was to diagnose caseous lymphadenitis in sheep by PCR assay . A total of 1257 sheep from different flocks in Dakahlia governorate were clinically examined during the period of January 2014 to February 2015. sheep were classified according to the status of infection in the flocks to infected , incontact and free ones. 37 needle biopsy samples were randomly collected from 26 diseased, 7 incontact and 4 free examined sheep for PCR assay. rpoB gene was detected in 37 samples (100%) while PLD gene was in 18 samples (48.64%), from which 16 from diseased sheep and two from incontact ones, while not detected in free sheep. The PCR assay was rapid, specific and significant technique as bacterial culture in detecting corynbacterium pseudotuberculosis in needle biopsy samples from sheep < /span>https://mvmj.journals.ekb.eg/article_126853_48e3ee6d273a34d6878d7f8312a2b70b.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PLAIN AND CONTRAST RADIOGRAPHIC EVALUATION OF THE DIGIT IN DONKEYS (EQUUS ASINUS)516512685610.21608/mvmj.2017.126856ENMohamedSalemDepartment of Surgery, Anesthesiology and Radiology, Fac. of. Vet. Medicine Mansoura UniversityEl-SayedEl-ShafaeyDepartment of Surgery, Anesthesiology and Radiology, Fac. of. Vet. Medicine Mansoura UniversityEsamMosbahDepartment of Surgery, Anesthesiology and Radiology, Fac. of. Vet. Medicine Mansoura UniversityAdel E.IZaghloulDepartment of Surgery, Anesthesiology and Radiology, Fac. of. Vet. Medicine Mansoura UniversityJournal Article20170912<span>Radiography of the digit is a golden standard technique allows the veterinarian to render a subjective evaluation of the digit in donkeys. The present study was planned to evaluate the bony tissues and blood circulation in donkey’s digit using plain and contrast radiography (angiography and venography). The digits of ten clinically and orthopedic healthy donkeys were subjected to plain and contrast radiographic examination of the digit region using four standard views (dorsopalmar, anterior-posterior, lateromedial and oblique views). Plain radiographic examinations revealed fully descriptions of the bony structures and joint surfaces of the donkey’s digits. While, the digital angiography and venography of the donkey digit illustrate clearly evident digital arteries and veins network that are course from the carpus/tarsus to the terminal arch. In conclusion, radiography provides a pierce non-invasive technique for evaluation of the hard tissue and digital blood circulation of the digit in donkeys.</span>https://mvmj.journals.ekb.eg/article_126856_d12edc9d8723fc2b67a4e58930be1c85.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA455012686410.21608/mvmj.2017.126864ENAmiraMostagerTheriogenology department, Faculty of Veterinary Medicine, Mansoura University, EgyptAbd El-MoneimMontaserTheriogenology department, Faculty of Veterinary Medicine, Mansoura University, EgyptMagdy RamdanBadrArtificial Insemination and Embryo Transfer Department, Animal Reproduction Research InstituteAbd El-Raouf OsmanHegabBiology Department, Faculty of Science, Taif University, KSASamy MoawadZaabelTheriogenology department, Faculty of Veterinary Medicine, Mansoura University, EgyptJournal Article20170912<br /><span>The aim of the present study to compare 4 different types of media, SOF, TCM-119, CR1aa and KSOM, for in vitro culture of buffalo embryo. This study was under taken in the Department of Artificial insemination and embryo transfer, Animal Reproduction Research Institute (ARRI), Al-Haram, Giza .The selected cumulus oocyte complexes(COCs) with a compact cumulus cells and evenly granulated ooplasm were in vitro matured at 38.5°C in atmosphere of 5% CO2 with high humidity .After 24h of culture, oocyte were used for in vitro fertilization . Following 18h of insemination, oocyte were randomly assigned to investigate the effect of different types of media from the zygote to day 7 post-insemination . there were no significant difference in cleavage rate among 4 media However, the morula and blastocyst stage increased significantly(P<0.05) in using SOF (26.03, 17.88% respectively) and also by using CR1aa (25.37, 16.42% respectively) compared with TCM (11.86, 5.08% respectively) and KSOM (9.62, 3.85 %respectively).There is no significant difference between SOF and CR1aa.In conclusion, the current data reveled that SOF is the best media used in embryo development in buffalo. Key words: SOF. In vitro culture media. Buffalo</span>https://mvmj.journals.ekb.eg/article_126864_8de050d043ab8ff7e1503e1cd98a9dab.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212THE EFFECT OF LIVERIA® ON SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF TREATED BUFFALO CALVES233212687110.21608/mvmj.2017.126871ENMohamed GEl-SayedPharmacology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptM MEl-DiastyAnimal health research institute, Mansoura, Proventional labReham AEl- ShafeiPharmacology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.Marwa SBarakatAnimal health research institute, Mansoura, Proventional labJournal Article20170912<span>The effect of intramuscular injection of Liveria® at a dose of 1ml/20kg body weight on hematological and biochemical parameters was investigated in fifteen apparently healthy buffalo calves of our local breed, 2-3 months age old, with average body weight 53-72 Kg in especial farm at Dakahlia Governorate. Buffalo were divided into three groups, each group five animals. The first group was injected with saline I/M (1ml/20kg) and lefted without any drug (the controlled animals). The second group was injected intramuscular by Liveria® (1ml/20 kg body weight). The third group was injected three successive intramuscular doses by Liveria (1ml/20 kg body weight) for three days. Hematological (RBCs- WBCs-Hb), biochemical aspects (total protein- albumin- globulin- serum: ALT, AST and ALP) and some minerals concentration (Ca, P and Na) were evaluated. The results showed that the administration of Liveria in calves caused a significant decrease in total erythrocytic count, haemoglobin concentration, total leukocytic count, total protein, albumin and serum AST with a significant increase also in serum calcium and inorganic phosphorus. Moreover, no changes in ALT, ALP activities and globulin level. Therefore, it was concluded that a single dose of Liveria® at dose rate (1ml /20 kg body weight) induced a significant improvement in some heamatological and biochemical parameters of treated calves</span>https://mvmj.journals.ekb.eg/article_126871_39ac16b172888d60d731d696dcaac5e4.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECTS OF DIETARY FAT SUPPLEMENTATION ON GROWTH PERFORMANCE OF EARLY WEANED RABBIT152112734410.21608/mvmj.2017.127344ENA MOrmaDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptHossam Eldin MAbd ElbakyDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptT IMohamedDept. of Nutrition & Nutritional Deficiency Diseases, Faculty of Vet. Med., Mansoura University, EgyptJournal Article20170912<span>The present study was carried out to evaluate the effect of dietary supplementation of soybean oil (SBO) to increase neutral detergent fiber (NDF) contents on growth performance (GP), nutritive values (NV) and carcass quality of growing New Zealand White (NZW) rabbits. A total sixty, 5 weeks old male weaned rabbits of mixed breeds with a mean body weight of 774.42g were randomly allocated to four dietary groups of 15 rabbits each. Experimental groups were fed diets supplemented with 0, 1, 2 &3% fat. The feeding trial lasted for 6 weeks. Growth performance parameters as body weight (BW), feed intake (FI) and feed conversion ratio (FCR) were determined every week. At the end of the experiment (8 weeks), 6 rabbits from each group were randomly taken, weighted and slaughtered to complete bleeding. The rabbits from each treatment were dressed, eviscerated samples taken for determination of moisture, CP, EE and ash contents. The obtained results revealed that supplementation of SBO in diets NZW rabbits up to 2% improve BW and FCR and didn’t significantly affect the feed intake. However, supplementation of 3% SBO in diets of NZW rabbits did not significantly increased the BW and weight gain (BWG), FI and FCR. Fat supplementation didn’t affect the dressing carcass percent & internal organs weight. The present study concluded that supplementation of SBO in diets NZW rabbits up to 2% significantly improve the growth performance and body composition.</span>https://mvmj.journals.ekb.eg/article_127344_e52cc937fcd5bced841bfbbdcdcc9af8.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212STUDIES ON H9 AVIAN INFLUENZA IN DAKAHLIA GOVERNORATE30131712734610.21608/mvmj.2017.127346ENAsmaa AAlzayatDepartment of Poultry Diseases, Animal Health Research Institute, Mansoura branch, EgyptAbdelfattah HEladlDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, EgyptHanan AFahmyDepartment of Biotechnology, Animal Health Research Institute, Dokki, Egypt.Hany FEllakanyDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Damanhour University, EgyptJournal Article20170912<span>Prevalence of H9 Avian Influenza Virus (AIV-H9) in Dakahlia governorate during 2012 - 2015 was undertaken to update the epidemiology of avian influenza virus infections among poultry flocks in Egypt. A total of 50 chicken flocks were examined (28 broiler flock and 22 layer flock). Results revealed that 18 samples were HA positive while 7 out of 18 samples were positive by chromatographic strip test for common antigen of AIV. Two chicken flocks were found positive AIVH9 by RT-PCR and qrt-PCR. The virus was successfully isolated and propagated in SPF eggs. The result was screened by HA and confirmed by chromatographic strip test, RT-PCR and qrt-PCR. The PCR tests confirmed that the 2 isolates (12B and 19L) were free from contamination with H5 and ND. Both isolates (12B and 19L) were titrated giving EID50 10 8.5 and 10 8. Pathogenicity index (PI) of the isolates (12B and 19L) was assessed experimentally 0.8 and 1.1. Experimentally, the results also revealed faster mortality occurred with both H9 infection and IB live strain vaccination. In conclusion, our results confirmed the continuous circulation of the AIV- H9 in chicken flocks in Dakahlia governorate which exaggerate the respiratory problems in poultry farms.</span>https://mvmj.journals.ekb.eg/article_127346_863504802aec7888b6c36fea26d8540c.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECTS OF MELATONIN ON TESTICULAR AND PITUITARY GLAND FUNCTIONS IN RATS59359412734710.21608/mvmj.2017.127347ENNabilAbu HeakalDepartment of physiology, faculty of veterinary medicine , Mansoura UniversityJournal Article20170912<span>The present investigation was desgined to evaluate the effects of melatonin on testosterone and pituitary hormones levels in mature male rats Twenty mature male rats were used in this study. Animal were divided into two groups each one consists of ten rats . The first group considers as contral group administrated soya oil (2ml / kg) , the second group treated with melatonin (0.5mg 1 kg) for one month . After the end of the experiment rats were sacrificed and blood was collected into heparinzed tubes to separate plasma and testicular tissue were collected to detect oxidive changes of the malenaldehyde (MDA) concentration and activities of superoxide dismutase (SOD) and catalase (CAT) . The results revealed that plasma level of testosterone and luteinzing hormones were significantly deceases (P ‹ 0.5) in melatonin treated group as compared with normal one , while reduces testicular concentration of MDA and increases the SOD and catalase activities . It was concluded that Melatonin has direct effects on testiculars and pituitary gland hormones and has antioxidant effects.</span>https://mvmj.journals.ekb.eg/article_127347_bc680cec2f89c315f486d2a1752605ff.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PHYSIOLOGICAL AND HISTOLOGICAL STUDIES ON THE EFFECT OF MYRTUS COMMUNIS EXTRACT ON RAT’S THYROID GLAND59159212735010.21608/mvmj.2017.127350ENNAl-ZailZoology Department / Faculty of science / Omar El-Mukhtar UniversityA MAboalhajZoology Department / Faculty of science / Omar El-Mukhtar UniversityS M.sEl-GharbawyCytology and Histology Department / Faculty of veterinary medicine / Cairo UniversityMAbd El-GawadAnatomy & Embryology, Department Faculty of Veterinary Medicine, Mansoura UniversityJournal Article20170912<span>The aim of this study was to test the effect of the ethanolic extract of Myrtus communis leaves on the thyroid gland tissue in white rats . The animals were given a dose of 2 g/kg orally. 43 rats were divided into 3 groups. The first group was given the dose daily for 7 days . The second group was treated for 14 days. The third group was given the dose for different hours , and after the end of each treatment period , blood samples were taken and the levels of thyroid hormones (T3 , T4) and thyroid stimulating hormone (TSH) were determined . Each group included some animals as a control. Some of the animals treated with the extract showed bleeding through mouth and nose , were lethargic , tired and lost appetite . Furthermore, the weight of these animals was significantly lower than those in the controls. The animals treated with the extract had a significant increase in the levels of TSH, T3 and T4. Thyroid gland of treated rats showed an increase in the size of thyroid follicles especially those found at the periphery of the gland . These follicles became irregular and distended by an accumulation of heterogeneous colloid with an increase in the amount of vacuoles at the periphery of this colloid. Moreover, some inflammatory cells appeared inside the colloid of some follicles. However, follicular cells appeared compressed with flattened nuclei. Some follicles with ill-distinct boundaries were also appeared. The aim of this study was to test the effect of the ethanolic extract of Myrtus communis leaves on the thyroid gland tissue in white rats . The animals were given a dose of 2 g/kg orally. 43 rats were divided into 3 groups. The first group was given the dose daily for 7 days . The second group was treated for 14 days. The third group was given the dose for different hours , and after the end of each treatment period , blood samples were taken and the levels of thyroid hormones (T3 , T4) and thyroid stimulating hormone (TSH) were determined . Each group included some animals as a control. Some of the animals treated with the extract showed bleeding through mouth and nose , were lethargic , tired and lost appetite . Furthermore, the weight of these animals was significantly lower than those in the controls. The animals treated with the extract had a significant increase in the levels of TSH, T3 and T4. Thyroid gland of treated rats showed an increase in the size of thyroid follicles especially those found at the periphery of the gland . These follicles became irregular and distended by an accumulation of heterogeneous colloid with an increase in the amount of vacuoles at the periphery of this colloid. Moreover, some inflammatory cells appeared inside the colloid of some follicles. However, follicular cells appeared compressed with flattened nuclei. Some follicles with ill-distinct boundaries were also appeared.</span>https://mvmj.journals.ekb.eg/article_127350_ec15cdf61e05a2ea78c6c00dfd6bf64f.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212STUDIES ON CURRENTLY CIRCULATING PARAMYXOVIRUSES IN PIGEONS10511512735410.21608/mvmj.2017.127354ENReham AElbhnsawyDepartment of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Mansoura University.K IAbu El AzmDepartment of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Mansoura UniversitySanaa SAwadDepartment of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Mansoura UniversityA HEladlDepartment of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Mansoura University.Journal Article20170912<span>Twenty two pigeon paramyxovirus (PPMV) isolates were recovered from 50 tested diseased pigeons. Tested pigeons suffered from nervous manifestation, listlessness, inappetance, inability to fly and greenish diarrhea. Diseased pigeons were collected from Dakahlia governorate during 2014 and 2015. Virus isolation was done in embryonated chicken eggs via allantoic sac inoculation. Isolates were identified as PMV by HA and RT-PCR tests. Pathogenicity for five isolates was done via intracerebral inoculation of one day old 10 SPF-chicks for each isolate. Intracerebral pathogenicity index varied from 0.0 to 1.44. Although RT-PCR testing of liver, spleen, lung and brain collected from culled chicks on day 8 post inoculation for group with 0.0 pathogenicity index and negative control group were negative, yet testing of the same organs from dead birds of other challanged groups were positive for PMV.</span>https://mvmj.journals.ekb.eg/article_127354_d9ed728131e7678ead689e331cd64a2c.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212GENETIC SELECTION OF SOME IMMUNOLOGICAL TRAITS IN LOCAL EGYPTIAN CHICKEN STRAIN24926112739010.21608/mvmj.2017.127390ENKh MEl-Bayomi*Department of Animal wealth Development, Fac. of Vet. Med., Zagazig Univ., EgyptF AAbd El-GhanyDepartment of Poultry Breeding, Anim. Prod. Res. Inst., Agric. Res. center, Giza, EgyptR ADarwishDepartment of Husbandry and Animal Wealth Development, Fac. of Vet. Med., Mansoura Univ., EgyptNagwan A. El-SayedAhmedDepartment of Poultry Disease, Anim. Health Res. Institute, Mansoura labJournal Article20170912<br /><span>This work was carried out to investigate the effectiveness of selection for high immune response against Newcastle Disease Virus (NDV) vaccine in local Egyptian chicken strain (Dokki4) for two generations. Two lines (selected and control) of Dokki-4 strain were used; in the selected line, chicks were selected according to its immune response against NDV vaccine. In the control line, chicks were randomly chosen. The population families were vaccinated against the common prevalent diseases according to the vaccination programs in the Sakha Animal Production Research Station, Kafr El-Sheikh. Results of selection for high immune response against NDV vaccine showed that; selection was efficient in native Egyptian chicken strain (Dokki-4) for increasing antibody titer levels in the high selected line when compared with the control line (P < 0.05) in two generations. Highly significant differences between generation and line were detected. Sex differences in immune response to NDV vaccine were significant (P < 0.05) for the overall averages a long all generations of selection. Female’s titers were higher than those of corresponding males of the same line and generation. Realized response per generation in the selected line was 16.76 and 60.18 for male and 18.54 and 69.50 for female in the first and second generations respectively. This study conducted that the cumulative response for high antibody titers responses against NDV vaccine was 76.94 for male and 88.04 for female after two generations of selection. There were significant differences in livability percentage between high and control lines for antibody response; where high line had higher livability than the control line. The high selected line showed a higher survival rate post challenge, with significant difference (P < 0.05) than the control group in the first week (92.86 % and 86.11 %) and the second weeks (87.69 % and 77.42 %) of observing vaccinated birds. It could be concluded that, genetic selection for high antibody titers against NDV vaccine in Dokki-4 strain improved immune response at different ages under investigation with increasing livability percentages and disease resistance of the birds through two successive generations of selection.</span>https://mvmj.journals.ekb.eg/article_127390_9f18cb3f0b9fc0236eebbe4d366f0c6b.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212A CASE OF PYTHIOSIS IN A DONKEY24324812739110.21608/mvmj.2017.127391ENEsamMosbahDepartment of Surgery, Anaesthesiology and Radiology, Mansoura University, Mansoura, EgyptWalaaAwadinDepartment of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptJournal Article20170912<span>A case of crater-shaped granulomatous ulcer was observed in skin covering knee joint of right forelimb in 8 years old male donkey referred to Mansoura veterinary teaching hospital. Clinical examination of the lesion showed necrotic tissue with tumor appearance, abundant fibrinous-bloody exudation and yellowish-white caseous material (kunkers). Mycological isolation revealed white to off-white colonies at 37°C but not at 25°C. The colonies did not present aerial mycelium, and the mycelial growth penetrated the medium in all cultures. Histopathologically, the lesion with the hematoxylin-eosin (H&E) stain showed severe pyogranulomatous dermatitis, with abundant eosinophilic and neutrophilic inflammatory infiltrate and poorly stained thin septated hyphae. Immunohistochemistry showed positively stained hyphae inside the lesion using rabbit polyclonal antibody. The definitive diagnosis of the disease was based on the clinical features, mycological isolation, histopathological findings and immunohistochemical staining. This report described a case of pythiosis in a donkey.</span>https://mvmj.journals.ekb.eg/article_127391_685ea385ab66d2d3969c1b53e396804c.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES23524212739210.21608/mvmj.2017.127392ENKhairy MEl-BayomiDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptIman EEl ArabyDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptAyman ASalehDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptHoda ZOsmanAnimal Production Research Institute (APRI), Agricultural Research Centre, Ministry of Agriculture, EgyptMahmoud SEltarabanyDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptAshrafAwadDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptAmir HAbd El-fattahDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, EgyptMohammedAbu El-MagdDepartment of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University.Journal Article20170912<span>Bone Morphogenetic Protein 15 (BMP 15) is a member of transforming growth factor-β (TGF- β) superfamily which plays an important role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes hiefers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (222 bp) and BMP15.2 (222 bp) which includes exon 2 of BMP 15 gene. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals. Further studies must be done on another loci on BMP 15 gene or other genes related to infertility in Egyptian buffaloes</span>https://mvmj.journals.ekb.eg/article_127392_0ede72b4e639232ce2d5b00eb8bd7fbb.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EVALUATION OF SOME MILK ENZYMES AS BIOMARKERS IN DETECTION OF BOVINE SUBCLINICAL MASTITIS55957112739310.21608/mvmj.2017.127393ENAAbdelKhalekFood Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University.A AEl-GamalAnimal Health and Research Institute. Mansoura branchShaimaaAAnimal Health and Research Institute. Mansoura branchJournal Article20170912<span>The present study was carried out on One hundred and fifity , one day old chicks to evaluate the immunogenicity of multivalent pilus vaccine containing pili from the three common pathogenic serotypes of E. coli (O1, O2 and O78). Nienty vaccinated birds at 14 days & 28 days of age by 0.5ml s/c of pilus vaccine at base of neck and 60 unvaccinated were divided to 4 subgroups (30 vaccinated & 20 unvaccinated) were used for challenge two weeks after second dose . Each group was challenged each with one type of E.coli strains O1 , O2 and O78. Blood samples were collected during the experiment from the flocks one week before vaccination and weekly for 7th week after the first dose of vaccination for evaluation of immune response by using ELISA test. The prepared multivalent E.coil pilivaccine from combination of O1, O2 and O78E.coli strains were seemed to cover good range of protection and has been elicited a protective immune response against virulent E.coli challenge with homologous and heterologous strains. strong correlation was found between antibody response vaccinated groups and low lesions score that indicated a good protection.</span>https://mvmj.journals.ekb.eg/article_127393_f40cb8fbcf091633ef7d0a6b28ef4cf5.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212IMMUNOLOGICAL STUDIES ON E.COLI VACCINE IN CHICKEN54955812739510.21608/mvmj.2017.127395ENAmany MAbd.ELEmoatyDep. of Bacteriology, Mycology and Immunology, Fac. Vet.Med, Mans. Univ.Ahmad M.AAmmarDep. of Microbiology, Fac. Vet. Med, Zagazig Univ.AlaaEldin HMoustafaDep. of Bacteriology, Mycology and Immunology, Fac. of Vet. Med. KafrElsheikh Univ.Ibrahim SIbrahimVaccines Research Institute,Abbasia,CarioJournal Article20170912<span>The present study was carried out on One hundred and fifity , one day old chicks to evaluate the immunogenicity of multivalent pilus vaccine containing pili from the three common pathogenic serotypes of E. coli (O1, O2 and O78). Nienty vaccinated birds at 14 days & 28 days of age by 0.5ml s/c of pilus vaccine at base of neck and 60 unvaccinated were divided to 4 subgroups (30 vaccinated & 20 unvaccinated) were used for challenge two weeks after second dose . Each group was challenged each with one type of E.coli strains O1 , O2 and O78. Blood samples were collected during the experiment from the flocks one week before vaccination and weekly for 7th week after the first dose of vaccination for evaluation of immune response by using ELISA test. The prepared multivalent E.coil pilivaccine from combination of O1, O2 and O78E.coli strains were seemed to cover good range of protection and has been elicited a protective immune response against virulent E.coli challenge with homologous and heterologous strains. strong correlation was found between antibody response vaccinated groups and low lesions score that indicated a good protection.</span>https://mvmj.journals.ekb.eg/article_127395_aa697d0abf30f1f6c796a6114414cadb.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212DETECTION OF OXYTETRACYCLINE RESIDUES IN EXPERIMENTALLY INJECTED CHICKENS from DAKAHLIA POULTRY FARMS, EGYPT54154712760810.21608/mvmj.2017.127608ENTamer M. M.GadEducational Veterinary Hospital, Faculty of Veterinary Medicine, Mansoura University, EgyptAlaa El-Din M. A.MorshdyFood Control Department, Faculty of Veterinary Medicine, Zagazig University, EgyptAdel I. M.El-AtabanyFood Control Department, Faculty of Veterinary Medicine, Zagazig University, EgyptJournal Article20170912<span>Antibiotics residues in chicken meat have a particular public health significance in the field of food safety due to its related resistance and emergence of antimicrobial-resistant microorganisms. The objectives of this study were firstly, to investigate the incidence of oxytetracycline residues in chicken muscles (pectoral and thigh) and its organs (liver and kidney) which were collected from Dakahlia poultry farms, Dakahlia governorate, Egypt. Secondly, to reduce these residues in chicken meat and its organs. The public health significance of oxytetracycline residues was discussed. Thigh muscles had the biggest inhibition zone in the examined samples on 1st and 2nd days after intramuscular injection of oxytetracycline in chickens followed by pectoral muscles, livers and kidneys. After that, inhibition zone reduced gradually in all examined samples on 3rd, 4th, 5th and 6th days till disappeared on 7th day from thigh and breast muscles together while on 9th and 10th days, it disappeared from livers and kidneys respectively. Both deep freezing at (-18:-20oc) for 14 successive days and boiling at 100oc for 60 minutes of examined samples are an efficient strategy in reducing oxytetracycline residues in chicken meat. Thus, strict instructions and precautions during using antibiotics for prophylaxis or treatment in poultry farms should be followed to reduce its residues in meat.</span>https://mvmj.journals.ekb.eg/article_127608_aeca65e468f5f8ed6e1f39630a736778.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ANTI-ATHEROSCLEROTIC ROLE OF OLIVE OIL IN RATS52153112760910.21608/mvmj.2017.127609ENBouzeedBouKhazeemDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityEl-SaidEl-SherbiniDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityGehadRamadanDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityMosbahAbdel GawadDepartment of Anatomy and embryology, Faculty of Veterinary Medicine. Mansoura UniversityJournal Article20170912<span>The increase in dietary cholesterol to perform a rat model for atherosclerotic diet would result in a significant increase in serum levels of total cholesterol and malondialdehyde and blood SOD activity in rats. Moreover, there was significant decrease in serum levels of LDL-C, triacylglycerol and GSH, In addition to blood catalase activity. Although, there was a nonsignificant change in in serum HDL-C and blood GPx activity. However, dietary supplementation with olive oil mixed with atherosclerotic diet ameliorates this undesirable effect .</span>https://mvmj.journals.ekb.eg/article_127609_5f4ce7924576d732ba8897e4193a6865.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ADVERSE EFFECTS OF LINCOMYCIN AND SPECTINOMYCIN IN RATS50351912761110.21608/mvmj.2017.127611ENS. AAmerPharmacology Department, Fac. Vet. Med, Mansoura UniversityReham A.APharmacology Department, Fac. Vet. Med,Enjy F.RClinical Pathology Department., Fac. Vet. Med., Mansoura UniversityHamza HMPharmacology Department, Fac. Vet. Med,Journal Article20170912<span>This study was performed to investigate the adverse effects of lincomycin (given orally at 500 mg/kg daily for 21 days) and spectinomycin (given S.C at 250 mg/kg daily for 30 days) on 24 apparently clinically healthy adult male albino rats (175 - 250 gm). Blood, serum and tissues (Liver, kidney and testis) samples were collected for hematological, biochemical and histopathological for investigation. The obtained results revealed that, lincomycin and spectinomycin treated groups showed a significant decrease in total erythrocytic and leucocytic count (especially lymphocytes), hemoglobin values and PCV value. The levels of ALT, AST, cholesterol, triglycerides and LDL revealed a significant increase in lincomycin and spectinomycin treated groups. While the level of HDL showed a significant decrease in both lincomycin and spectinomycin treated groups. The results also showed that, both drugs induced histopathological alterations in the liver, kidney and testis.</span>https://mvmj.journals.ekb.eg/article_127611_ac5357144b35d87b36f68aae19ddc46a.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212GENOTOXICITY OF BISPYRIBAC SODIUM IN ALBINO RATS49150212761210.21608/mvmj.2017.127612ENFathy RSleemprof. dr. of forensic medicine and toxicology Faculty of veterinary medicine, Mansoura University, Egypt.Mahmoud M.ElalfyLecturer of forensic medicine and toxicology Faculty of veterinary medicine, Mansoura University, Egypt.Mohamed S.Abou-mosallamDemonstrator of forensic medicine and toxicology Faculty of veterinary medicine, Mansoura University, Egypt. Faculty of veterinary medicine, Mansoura University, EgyptJournal Article20170912<br /><span>Genotoxicity of pesticides constitute great concern that affect population health, commercial formulation of such pesticides may increase the genotoxicity of certain pesticides, the genotoxic effect of bispyribac sodium had been studied in albino rats given orally 65.88, 32.94 and 16.47 mg / kg of bispyribac sodium twice per week for 3 months and the results indicated that significant increase in chromosomal aberration (break, fragment, gap, ring and chromosomal association) in respect to control which was dose dependent also significant increase in DNA quantity, also there was significant increase in micronuclei level in respect to control and decrease in mitotic activity in bone marrow cell where the mitotic index had decreased drastically in response to control value and these results confirm that bispyribac sodium may have genotoxic effect affect in albino rats.</span>https://mvmj.journals.ekb.eg/article_127612_dc2b35fc4bcac888489d4449cdba005c.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212SOME PHARMACOLOGICAL STUDIES ON MARBOFLOXACIN AND FLUNIXIN MUGLUMINE COMBINATION DURING TREATMENT OF COLIFORM MASTITIS IN CATTLE48148912761610.21608/mvmj.2017.127616ENM. GEl-SayedPharmacological Dep., Faculty of Veterinary Medicine, Mansoura UniversityMohamedEl-DiastyAnimal Health Research Institute – Mansoura provincial lab.M MShamsAnimal Health Research Institute – Mansoura provincial lab.Journal Article20170912<span>The aim of the present work was to study the effect of Marbofloxacin with Flunixin Muglumine combination during treatment of coliform mastitis in cattle. Twenty coliform mastitic cows were selected from 50 mastitic cows and treated by Marbofloxacin and Flunixin muglumine. The results showed that the red blood cell (RBC) count and hemoglobin concentration were significantly decreased. Similar changes were observed with the mean corpuscular volume and total platelets. Meanwhile, White blood cell (WBC) count was significantly elevated. There are no significant changes recorded in serum albumin and globulin with tested drugs throughout the study. However, there is a significant increase in serum total proteins. The result recorded also, a significant increase (in serum aspartate aminotransferase (AST) and serum alkaline phosphatase (ALP)) and a significant decrease in serum alanine aminotransferase (ALT) was recorded</span>https://mvmj.journals.ekb.eg/article_127616_6361b37b39b7ec85fa67d794e303de4f.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212MOLECULAR IDENTIFICATION OF E. COLI AND SALMONELLA SPECIES ISOLATED from FRESH WATER FISH BY POLYMERASE CHAIN REACTIONS (PCR)46748012761810.21608/mvmj.2017.127618ENViola H.ZakiProfessor of Fish Diseases and Management. And Head of Internal Medicine, Infectious Diseases and Fish Department Faculty of Veterinary Medicine, Mansoura University,Adel H.El-GoharyProfessor of Zoonoses and Head of Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University,Mostafa M.NabihLecurer of fish disease, Department of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, Saudi Arabia.Journal Article20170912<span>Bacteriological examination of 400 fish samples taken from Oreochromis niloticus (O. niloticus), Clarias gariepinus, Cyprinus carpio.l (Common carp) and Mugil cephalus (100 from each) revealed isolation of (317) bacterial isolates, which were identified as 171 E.coli isolates and 146 Salmonella sp isolates with an incidence of (53.94% and 46.06 %) respectively. The incidence of E.coli and Salmonella spp. differ among different species of fish under investigation, the higher percentage of bacterial isolates observed in Clarias garipinas (53.31%), followed by (29.97 %), Cyprinus carpio.l (11.99 %), and the lower incidence was observed in Mugil cephalus (4.73%). Frequency distribution of isolated bacteria from the surface lesions and internal organs of examined fish and the antibacterial susceptibility to different chemotherapeutic agents were discussed. PCR based technique was used in identification of E. coli and Salmonella sp., the results showed that E. coli O26 & O111 were positive strains for stx1, stx2 and eaeA genes, E. coli O55 was positive strain for stx2 and eaeA genes, E. coli O91 & O103 were positive strains for stx1 and stx2 genes, E. coli O119 was positive strain for stx2 gene, E. coli O124 was positive strain for eaeA gene and E. coli O128 was positive strain for stx1 gene. In case of Salmonella sp, S. Typhimurium strain was positive for stn, invA and hilA genes, S. Enteritidis strains was positive for stn, invA and hilA genes, S. Virchow strain was positive for invA gene, S. Typhi strain was positive for invA and hilA genes and S. Infantis strain was positive for invA genes</span>https://mvmj.journals.ekb.eg/article_127618_cab7883299d9baa277d851b975dc68fd.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212ROLE OF LYSINE AND METHIONINE IN CARNITINE METABOLISM IN RATS45146612762410.21608/mvmj.2017.127624ENAliaaEzz El-DienDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary medicine, Mansoura UniversityYoussefMoustafaDepartment of biochemistry ,faculty of veterinary medicine, Zagazig UniversityMohamedEl_AdlDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary medicine, Mansoura UniversityAmrHassanDepartment of Nutrition, Faculty of Veteterinary Medicine, Mansoura UniversityJournal Article20170912<span>The present study was performed on rats for studying effect of different concentrations of lysine and methionine in carnitine metabolism in liver and muscle tissues. The animals which fed low concentrations of lysine and methionine showed significant decrease in carnitine metabolism when compared with normal control group. On the other hand the rats supplied with high concentrations of lysine and methionine showed markedly increase in carnitine metabolism. Furthermore, this study clearly appear the role of lysine and methionine concentration in antioxidant status of the body</span>https://mvmj.journals.ekb.eg/article_127624_7ff932db8f12f2728da82f6a7981c814.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212SPECTROPHOTOMETRIC QUANTIFICATION OF NITRITE AND NITRATE IN CURED PROCESSED MEAT43944912762610.21608/mvmj.2017.127626ENM. M. M.IElgazzarDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University.Z. K.M.Al-AwamryRegional Center for Food and Feed, Agricultural Research Center.H. A. M.MaroufFood Inspection Laboratory, Animal Health Research Institute.N. H. M.KhalilFood Inspection Laboratory, Animal Health Research InstituteJournal Article20170912<span>A total of 115 samples of different cured meat products – locally processed and imported ones – were purchased from various groceries and supermarkets in Egypt then analyzed for their contents of both nitrite and nitrate by the aid of spectrophotometer and expressed as mg/kg (ppm). The surveyed samples consisted of 25 corned beef (9 locally processed plus 16 imported ones), 20 locally processed beef sausage, 15 locally processed patirma, 10 locally processed beef luncheon, 5 imported canned beef luncheon, 20 canned chicken sausage (8 locally processed and 12 imported ones), 10 imported canned chicken luncheon, in addition to 10 locally processed chicken luncheon samples. Spectrophotometric analyses quantified the range (minimum – maximum) &mean ± S.E. levels of nitrite in the tissues of locally processed and imported corned beef samples as 4.35 – 43 & 14.59 4.13 mg/kg and 4.07 - 78.37 & 38.94 6.98 mg/kg, respectively, while similar findings for the nitrate were 13.91 - 40.58 & 22.8 2.64 mg/kg and 12.32 - 102.5 & 43.88 6.62 mg/kg in the same samples, consequently, the sum of both nitrite and nitrate residues were calculated as 23.91 - 62.2 & 37.39 4.5 mg/kg in locally processed corned beef besides 30.77 - 141.9 & 82.82 9.2 mg/kg in imported corned beef samples, consecutively. The aforementioned analytical technique exhibited the minima, maxima, and mean S.E. values of nitrite 6.65, 82, 28.72 4.34 mg/kg; of nitrate 8.93, 113.01, and 40.6 5.94 mg/kg; of sum nitrite and nitrate 16.49, 138.7, and 69.33 7.92 mg/kg, respectively, in the tissues of locally processed beef sausage. While, those findings were 2.03, 69, and 22.35 4.96 mg/kg for nitrite; 0.76, 114.25, and 54 10.42 mg/kg for nitrate; as well as 18.99, 142.71, and 76.35 10.06 mg/kg for sum nitrite and nitrate, successfully in the samples of locally processed pastirma. The lowest levels of nitrite, nitrate, and sum nitrite and nitrate in the analyzed tissues of both locally processed beef luncheon and imported canned beef luncheon samples were 5.22 & 36.73, 1.99 & 2.99, and 18.99 & 63.29 mg/kg ; whereas the highest amounts of such residues were 75.5 &75.78, 74.7 & 59.56, and 131.7 & 96.29 mg/kg ; meanwhile the mean levels S.E. of these findings were 30.63 8.28 & 59.22 8.32, 32.81 6.4 & 21.78 11.16, and 63.43 12.46 & 81 6.81 mg/kg in the same samples, successfully. Tissues of locally processed canned chicken sausage revealed nitrite nitrate, and sum nitrite/nitrate by levels ranged from 7.46 – 47.08, 7.5 - 44.8, and 22.06 - 91.88 mg/kg with mean S.E. values 27.54 4.54, 16.22 4.6, and 43.77 7.96 mg/kg, respectively. While, those findings in surveyed samples of imported canned chicken sausage were 18.46 – 64.11, 2.65 – 93.78, 43.51 – 132.08 with mean S.E. values 39.21 3.61, 29.07 7.36, and 68.28 7.07 mg/kg, consecutively. The mean S.E. levels and ranges (minimum – maximum) of the investigated analytes in the samples of imported canned chicken luncheon were found 45.86 9.37 and 21.69 - 99.52 mg/kg for nitrite, 49.55 14.55 and 12.65 - 149.76 mg/kg for nitrate, in addition to 95.43 16.03 and 46.59 - 198.36 mg/kg for sum nitrite/nitrate, respectively. Meanwhile, similar findings in the tissues of locally processed chicken luncheon were 35.96 5.55 and 11.21 - 58.4 mg/kg for nitrite, 37.92 9.05 and 1.06 - 99.81 for nitrate, as well as 73.88 6.94 and 47.36 - 120.98 mg/kg for sum nitrite/nitrate, successfully. An overviewing the obtained results, none of surveyed samples of cured meat products possessed nitrite or nitrate level more than their limits (150 & 250 mg/kg, respectively). Also, public health significance of nitrite and nitrate in cured meat products – marketed in Egypt – as well as the conclusion and recommendations of the present study were also mentioned.</span>https://mvmj.journals.ekb.eg/article_127626_1d70274429a934cd9482011b2487d530.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PREVALENCE OF CAMEL BRUCELLOSIS AT AL-SHALATEEN AREA334412763910.21608/mvmj.2017.127639ENA. MEl-SayedAnimal Health Research Institute, Al-Shalateen Provincial LabM. M.El-DiastyAnimal Health Research Institute, Mansoura Provincial Lab.M. A.ElbeskawyAnimal Health Research Institute, Mansoura Provincial LabM.ZakariaInternal medicine and Infectious. Disease Dep. Fac. Vet. Med., Mansoura UniversityE. E.YounisInternal medicine and Infectious. Disease Dep. Fac. Vet. Med., Mansoura UniversityJournal Article20170912<span>Sera of 801 apparently healthy dromedary camels were collected from Al-Shalateen quarantine and tested by BAPAT, RBPT and there results were confirmed by CFT. The results revealed that the prevalence of camel brucellosis was 12.9%, 11.6% and 11.5% for BAPAT, RBPT and CFT respectively. Also results revealed that seroprevalence was higher in camels younger than 2 years (immature) (13.3%) than mature camels (2-4 years old) (10.6%) and camels at breeding age (older than 4 years) (10.8%). In addition, prevalence on females (19.1%) were higher than males (7.1%). Moreover, Brucella melitensis biovar 3 was isolated from stomach content of aborted camel fetus. Statistical analysis of these results revealed that the apparent prevalence (AP) was estimated as (11.5%) while true prevalence (TP) was estimated as (13.6%) (95%CI: 11.2-16%). There is No significant difference were detected between different age groups while a highly significant difference were detected between season and gender in the frequency of +ve and –ve samples in different tests.</span>https://mvmj.journals.ekb.eg/article_127639_364a786b9df0ce6ddf8eb908db3fbd44.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212HEAVY METAL RESIDUES IN SOME MANZALA LAKE FISHES11412764910.21608/mvmj.2017.127649ENSamir MohammedAbd-ElghanyFood Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura UniversityRana Abd-ElnaserAbu El-Naase*Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura UniversityKhalid IbrahimSallamFood Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura UniversityJournal Article20170912<span>This study aimed to determine the content of heavy metals as lead, mercury, cadmium and arsenic in edible muscles of three common freshwater fishes like Nile tilapia, Flathead grey mullet and African catfish. A total of 300 freshly caught fish samples (100 each) of different weights collected from Manzala Lake, Egypt during both summer and winter seasons of 2012 have been analyzed using air acetylene flame atomic absorption spectrophotometer. The obtained results revealed that the presence of lead, mercury, cadmium and arsenic in all (100%) examined samples by means of 0.704 µg/g, 0.635 µg/g and 0.64 µg/g for Pb, 0.045 µg/g, 0.0145 µg/g and 0.017 µg/g for Hg, 0.025 µg/g, 0.006 µg/g and 0.020 µg/g for Cd and 0.511 µg/g, 0.621 µg/g and 0.568 µg/g for As in Nile tilapia, Flathead grey mullet and African catfish , respectively. heavy metal concentrations were significantly varied within and between the studied fishes. However, a significant correlation among heavy metals was observed. This study indicated that various concentrations of heavy metals exist in sampled fish species and most of those levels are within the maximum permissible limits proposed by the Egyptian standards except for Pb, its level exceeded the permissible limits in the majority of tested samples. Therefore, fish caught from Manzala Lake can be considered unsafe for human consumption</span>https://mvmj.journals.ekb.eg/article_127649_c6caff00f496226ec0f4a452ecaa277b.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212PREVALENCE AND VIRULENCE PROFILE OF AVIAN PATHOGENIC E.COLI ISOLATED from CLINICALLY DISEASED BROILER CHICKEN41943012767110.21608/mvmj.2017.127671ENGamalYounisBacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.AmalAwadBacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, EgyptSamahSamirBacteriology, Mycology and Immunology Department Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, EgyptJournal Article20170912<span>Escherichia coli plays a part in maintaining intestinal physiology. Although, there are pathogenic strains that cause different syndromes of diarrheal disease. E. coli strains are the cause of a number of diseases in broiler chickens, leading to death, decrease in production or condemning of carcasses. This study aimed to determine phenotypes and virulence associated genes of avian pathogenic Escherichia coli (APEC) recovered from diseased and freshly dead chickens including, shiga toxin 1, shiga toxin 2 and intimin using genotypic methods. A total of 200 chicken visceral organs (liver, lungs, spleen and heart) collected from diseased and freshly dead birds (four organs per each bird) were subjected for conventional culturing, serotyping and molecular CHARacterization of virulence genes. 32 (16%) of isolates were biochemically identified as E.coli and confirmed by PCR using phoA gene. O78, .O8, O145 and O1 are the most prevalent serotypes from APEC isolates. stx1 and stx2 were determined by multiplex PCR , while eae gene was detected by uniplex PCR . stx2 gene was detected in 50% ( 16/32), while, 81.26% (26/32) of APEC isolates harbored eae. stx1 was not detected in all the tested isolates. The obtained results demonstrated the importance of studies in E. coli of avian origin in regions associated with intensive poultry industry, aiming to evaluating the predominant strains and also acquiring preventive measures to minimize losses due to APEC</span>https://mvmj.journals.ekb.eg/article_127671_8d0c12dbdc89c777a1541702674d285a.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212DETECTION FOR THE MOST SUITABLE ANTIBIOTIC AND DISINFECTANT FOR PSEUDOMONAS AERUGINOSA ISOLATED from PNEUMONIC AND APPARENTLY HEALTHY FARM ANIMALS40541712767610.21608/mvmj.2017.127676ENNeermin. A.IbrahimDep. of Bacteriology, Mycology and Immunology, Fac. Vet. Med, Mans. Univ.YasserElnakerDep. of animal medicine, (Infectious Diseases) Fac. Vet. Med. Assiut UniversitySalwa MHDep. of Bacteriology, Mycology and Immunology, Fac. of Vet. Med. Kafrelsheikh Univ.Ahmed. M. A.A.Dep. of Microbiology, Fac. Vet. Med, ZagazigUniv.Journal Article20170912<span>The present study was carried out on 550 samples (nasal, tracheal swabs and lung tissues) collected from pneumonic and apparently healthy cows and buffaloes. The bacteriological examination revealed that Pseudomonas aeruginosa was isolated from 172(41.1%) out of 419 cow’s samples and from 46(35.1%) out of 131 buffalo’s samples. from 172 cow’s isolates there were 144 isolates related to serotype (1) and 28 isolates were untypable while buffalo’s positive isolates revealed that 40 isolates were belonging to serotype (1) and 6 isolates were untypable. The effect of disinfectants as phenol, formalin and ethyl alcohol on viability of tested strains showed that 3.0% phenol could kill all tested strains after 5 minutes, but 3% concentration of formalin has a marked killing effect on the growth of P. aeruginosa within 10–15 minutes, while 40% ethyl alcohol dilution could kill P. aeruginosa after 10 minute. Forty P. aeruginosa isolates were tested for antibiotic susceptibility to 12 chemotherapeutic agents. The most effective antibiotic was gentamycin (95.0%) then streptomycin (87.5%) and amikacin (75%), all tested strains were completely resistant to tetracycline, cephalothin and ampicillin. It is concluded that P. aerouginosa is one of the most important cause of pneumonia in cows and buffaloes also it resists many disinfectants and remain viable except by using appropriate concentration. In vitro antibiotic sensitivity test showed that most strains were susceptible to gentamycin and amikacin although they resist many types of antibiotics.</span>https://mvmj.journals.ekb.eg/article_127676_f3f9855256d8beec69f59fc42cd5661b.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212MOLECULAR DETECTION AND SEROTYPING OF FOOT AND MOUTH DISEASE VIRUS IN TONGUE EPITHELIUM SAMPLES39740412770310.21608/mvmj.2017.127703ENA. A.El-KenawyDepartment of Virology, Faculty of Veterinary Medicine , Mansoura University. Phone: +20-50-2372593, Fax: +20-50-237995E. E.YounisDepartment of Internal Medicine, Infectious and Fish diseases, Faculty of Veterinary Medicine, Mansoura University.Hiam M.FakhryVeterinary Serum and Vaccine Research Institute, FMD Department, Abbassia, Cairo, EgyptR.KaramDepartment of Virology, Faculty of Veterinary Medicine , Mansoura University. Phone: +20-50-2372593, Fax: +20-50-2379952Journal Article20170912<span>The aim of the present study is molecular identification and typing of FMDV circulating in Dakahlia governorate in Egypt, 2014. The study was accomplished on pooled nine tongue epithelium samples collected from diseased cattle and buffaloes expected to be infected with FMDV. The diseased animals showed fever , excessive salivation and ulceration on tongue and gum. Conventional reverse transcription polymerase chain reaction (RT-PCR) was used for identification of FMDV using universal primers. Then serotyping of the identified virus was done using RT-PCR for serotype O and A and Real time RT-PCR for serotype SAT2 . FMDV was identified in all the examined nine samples. Five of them were of serotype O, two of serotype A, one of serotype SAT2 and one sample was neither O, A nor SAT2. In conclusion, molecular techniques are sensitive and rapid methods for FMDV detection. In addition, serotypes O, A and SAT2 are circulating in Dakahlia Governorate, Egypt, 2014.</span>https://mvmj.journals.ekb.eg/article_127703_a9faa829914fb2ccd60095e45fa07155.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECTS OF BLINDFOLD AND TAIL BEND OF EGYPTIAN WATER BUFFALO ON BEHAVIOURAL REACTIVITY AND PHYSIOLOGICAL RESPONSES TO PAIN INDUCTION38139612795710.21608/mvmj.2017.127957ENRady A.MohamedDepartment of Hygiene and Preventive Medicine, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.Usama A.Abou-IsmailDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, Egypt0000-0003-3080-6999MustafaShukryDepartment of Physiology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, EgyptAhmed M.ElmoslemanyDepartment of Hygiene and Preventive Medicine, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.MohamedAbd-ElmagedDepartment of Animal Medicine, Faculty of Veterinary Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.Journal Article20170912<span>Buffaloes are an important economic source for milk and meat production and for work and draught power in Egypt. Improving management practices applied to water buffalo may not only improve their welfare but has also an economic benefit. This experiment was carried out to determine the effect of blindfold and tail bending during restraint for veterinary treatment of Egyptian buffaloes on their behavioural reactivity and physiological responses to stress. Forty-eight Egyptian water buffalo bulls, naïve to the testing situation, were arbitrarily assigned to either blindfold (visual restriction) (BF), tail bending (physical control) (TT) or control (CT) (no visual restriction or physical control) treatment during restraint. Animals were entered the squeeze chute and the sides of the chute were adjusted to make contact with the animal body so that preventing unsteady movements, and were subjected to a 3day (day 1, 2 and 3) testing trials (injection stress) of three minutes each (1 m pre injection induction phase, 1 m injection induction phase and 1 m post injection induction phase). Heart rate (HR) was recorded using a stethoscope as soon as the animal was positioned in the squeeze chute and the respiratory rate (RR) was determined through counting the movement of flank region, and various reactivity-indicating behaviours were collected onto check sheet. Average HR decreased in both TT and BF bulls during and after stress (P= 0.001) but the reduction in HR was greater in BF bulls (67.25±3.41 and 55.00±1.08, respectively) in day 3 after stress (P= 0.001). Similarly, average RR decreased in both TT and BF bulls (P= 0.001) during and after stress but the reduction was greater in BF bulls during stress (25.38±0.53 and 19.92±0.33, respectively). Blindfolding and tail bending buffalo bulls decreased average frequency of their behavioural indicators of reactivity including: chest chute forcing prestress (P=0.01), during and post stress (P=0.001); head move (P=0.001); kicking prestress (P=0.05) during stress (P=0.001) and post stress (P=0.05); struggling move prestress, during stress (P=0.001) and post stress (P=0.05). The reduction was greater in BF bulls in case of chest chute prestress (0.08±0.05 and 0.50±0.16, respectively), struggling move during (0.33±0.02 and 0.75±0.04, respectively) and post stress (0.13±0.06 and 0.29±0.08, respectively). Taken together, both tail bend and blindfold water buffaloes decreased behavioural and physiological indicators of stress but BF appeared more beneficial and may therefore be recommended to reduce stress accompanying routine veterinary examination of buffaloes</span>https://mvmj.journals.ekb.eg/article_127957_479d7dd0a0338fa8522ea707478f1d6d.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212DIURNAL DISTRIBUTION OF LAMBING IN SHEEP AND ITS RELATION WITH BEHAVIOUR AND SURVIVAL OF THE NEONATE LAMB36538012795910.21608/mvmj.2017.127959ENRagab A.DarwishDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, EgyptUsama A.Abou-IsmailDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, Egypt0000-0003-3080-6999Journal Article20170912<span>A total of 348 Finnish Landrace – Rahmani crossbred ewes were used in this study to show the diurnal distribution of lambing and its relation with behaviour and survival of the neonate lamb. At birth, based on lambing distribution at twelve-hour periods, ewes were allotted into either day light time group which included ewes that lambed during the day light hours (from 6.00 am to 6.00 pm) or night time group which included ewes that lambed during night hours (from 6.00 pm to 6.00 am). Data recorded included time of birth, neonatal behavioural progress, body temperature of neonatal lambs over the first 3 days of life and lamb mortality rates from birth to weaning. Significantly more ewes lambed during the night time than throughout the day light hours. Neither litter size nor birth weight of lambs had a significant effect on time of parturition. However, ewe parity had a significant effect on lambing time. With respect to neonate lambs, night time born lambs were less active at birth and denoted a decrease in their body temperature over the first 3 days of life with higher mortality rates from birth until weaning specifically during the first week of birth compared to day light born lambs. It could be concluded that, more lambing occurred during the night time than during the day light time. Litter size and birth weight of lambs did not appear to affect the time of parturition, whereas significant differences in lambing time were observed with regard to parity. Moreover, exposure to cold disrupted the behavioural patterns of the neonate lambs, decreased their ability to maintain body temperatures and increased mortality rates.</span>https://mvmj.journals.ekb.eg/article_127959_1d40ecc21ab1467c679624c737d12354.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212AMELIORATING EFFECTS OF CYSTEINE, GLUTATHIONE AND TAURINE DURING FREEZABILITY OF RAM SPERMATOZOA28929912796110.21608/mvmj.2017.127961ENAyat Abd El- MaksoudMohamedArtificial Insemination And Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram , Giza, EgyptFahim El Bahy El SayedWehaishPhysiology Department , Mansoura University, EgyptMona HamedShakerArtificial Insemination And Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram , Giza, Egypt.YoussefEl-SeadyPhysiology Department , Mansoura University, EgyptJournal Article20170912<span>Ram sperm have a higher ratio of polyunsaturated/saturated fatty acids and a lower ratio of cholesterol/phospholipids molar than other species, which renders the sperm much more vulnerable to oxidative damage caused by reactive oxygen species (ROS) .The aim of the current study was to evaluate the effects of cysteine, glutathione and taurine on the freezability of ram spermatozoa and biochemical parameters. Semen samples from ten mature mixed Barki rams were used in this study. Pooled semen samples were divided randomly into 7 aliquots one was diluted with a Shotor extender as a control basal diluent while others in addition to basal extender L-Cysteine, L- reduced glutathione and taurine were added by two levels for each to present seven experimental groups. Diluted semen cooled to 5oC then frozen by liquid nitrogen in 0.25 ml French straws. After 48 hr frozen, straws were then thawed individually at 37 oC for 30 sec. in a water bath then subjected to evaluation. The obtained results showed that, addition of glutathione by a rate of 5mM was significantly increases motility than control group. Similarly, addition of taurine by a rate of 50mM significantly increased the viability index of cryopreserved semen samples. Percentages of acrosomal defects were dramatically decreased (P < 0.05) with glutathione (10 mM) addition compared to control (9.00 ± 1.16 % and 21.00 ± 0.58 % respectively) In regards to sperm membrane integrity, cysteine (5 mM) provided a greater protective effect (47.00 ±1.16) compared to control (%, 35.33 ± 0.88 %). Antioxidant addition showed decreased enzyme leakage and significantly (P < 0.05) improved total antioxidant capacity (TAC) especially taurine (50Mm) compared to control group. In regards to DNA integrity, taurine (50mM) provided a significant protective effect (98.51± 0.42 %) compared to control. Findings of this study showed that addition of cysteine, glutathione and taurine to semen extenders, of greater benefit to quality of frozen-thawed ram sperm. However further investigation on their effect on fertilizing capacity and pregnancy rate still needed.</span>https://mvmj.journals.ekb.eg/article_127961_061e63b05e53d6f6d301adcaff538032.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212MYXOSPORIDIOSIS IN OREOCHROMIS NILOTICUS; MOLECULAR IDENTIFICATION AND OXIDATIVE STRESS BIOMARKERS34736312797910.21608/mvmj.2017.127979ENNevien KAbdelkhalekDepartment of internal medicine, Infectious diseases and fish diseases and management, faculty of veterinary medicine, Mansoura UniversityM. A.El-AdlDepartment of biochemistry and chemistry of nutrition, faculty of veterinary medicine, Mansoura UniversityM. F.HamedDepartment of pathology, faculty of veterinary medicine, Mansoura UniversityM. A.Al-ArabyDepartment of parasitology, faculty of veterinary medicine, Mansoura UniversityJournal Article20170912<span>Myxosporidiosisis a protozoan parasitic fish disease that causes damages to fish organs and might eventually end with mortalities and deterioration of meat quality of the affected fish. The presence of parasitic infestation is usually accompanied with the production of reactive oxygen and nitrogen species that produces extra damage to host organs and inactivation of antioxidant defense system that increase the intensity of parasitic infestation. Recently, the response of the infested tissue to oxidative stress is varieddepending on the type of parasite; therefore the use of oxidative stress markers as indicator for myxobolus infestation is relevant. In the present study, Oreochromis niloticus was investigated for oxidative stress markers (GST, SOD, NO., H2O2 and MDA) in liver, muscle, spleen and gills infested with myxobolus spp. Histopathological examination was performed to clarify the cellular damageassociated withmyxobolus infestation. Results indicated that there was a non-significant change in GST activity in all organs, while SOD activity was significantly decreased in liver and gills after myxobolus infestation. The concentration of nitric oxide, hydrogen peroxide, and lipid peroxidation were significantly increased in (gills and spleen);(liver, spleen and muscle); (liver and muscles), respectively. Intestine and eyes of the affected fish showed a degenerative changes and replacement of the epithelial tissues with myxobolus spores with CHARacteristiceosinophilic granules that has a role in the immunity of host against parasitic infestation.The phylogentic analysis showed that the identified species of myxobolusin the present study was revealed a close relationship as well as a high identity percentage with, Myxoboluskisutchi and similar tissue tropism which highlighted the use of both molecular identification and oxidative stress markers for diagnosis of myxobolus infestation in dead fish when the parasitological examination alone becomes inadequate foraccurate detection of myxobolus infestation.</span>https://mvmj.journals.ekb.eg/article_127979_197cfe3e63709ba0cd93e1fe7b102b6e.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212EFFECTS OF CALCIUM SALT OF FATTY ACIDS ON GROWTH PERFORMANCE AND BIOCHEMICAL PARAMETERS IN BROILER CHICKEN20922012798210.21608/mvmj.2017.127982ENRawda Abd-ElhayGadoBiochemistry Department, Vet. Medicine College, Mansoura University, EgyptG. REl-sayedBiochemistry Department, Vet. Medicine College, Mansoura University, Egypt.Mahmoud G.ElsebaaiBiochemistry Department, Vet. Medicine College, Mansoura University, Egypt.Journal Article20170912<span>The aim of this study is to determine if calcium salts of fatty acids (CSFA) can replace soybean oil in broilers diet. So 60 sex mixed Cobb chicks 10 day old divided into 3 groups, each included 20 chicks, and subdivided into 2 subgroups each included 10 chicks. All fed the same starter diet up to 10 days of age then fed grower and finisher diet with different dietary fat sources. Groups were: G1: basal diet with CSFA (Poly fat®) (PF), G2: basal diet with soybean oil (SO), G3: basal diet with Poly fat® + soybean oil 50:50 (PF:SO) at grower period, and 75:25 (PF:SO) at finisher period. Adding CSFA alone to broilers diet lead to a non significant decrease in the live body weight and feed conversion ratio (FCR)= 2, G3 had the best FCR=1.8 with a non significant increase in live body weight. CSFA showed a significant decrease in malondialdehyde (MDA) (P≤0.05), a non significant decrease in the level of blood total cholesterol, HDL Cholesterol and LDL Cholesterol, this was supported by gene expression of cholesterol carrier protein that showed a decrease in its expression in G1 and G3 when compared with control group but there was a significant decrease in blood triacylglycerol in G1 when compared with G3 (P≤0.05) that showed the lowest results in blood total cholesterol, there was a significant increase in reduced glutathione (GSH) content in blood (P ≤0.001) and liver tissue (P≤0.05) and a non significant increase in glutathione peroxidase activity in both blood and liver tissue in PF group when compared with control group, adding CSFA had no effect on both blood calcium and inorganic phosphorus. Conclusion: CSFA helps in reducing lipid peroxidation, oxidative stress and cholesterol level in broiler chicks.</span>https://mvmj.journals.ekb.eg/article_127982_1a5ac639912df6f47da96ec72d50c42d.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212MOLECULAR BIOLOGICAL STUDIES ON THE EFFECT OF GREEN TEA ON OBESE RATS22123412798610.21608/mvmj.2017.127986ENAsmaaNabihDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityIbrahim F.HassanDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityKhaled Abdel-AleimKahiloDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityMahmoud G.El-SebaeiDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityMohamed A.El-AdlDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine. Mansoura UniversityJournal Article20170912<span>The current study was conducted on 70 male albino rats for elucidation the role of different green tea either local (E) or imported (C) and capsulated like green tea (CAP) as well as two common active principle Epigallocatechin gallate (EPI) and polyphenols (POL) on high fat ration (HFR) fed rats. The results showed that EPI and POL were showing a significant improvement in lipid profile and anti-oxidant status of rats. Meanwhile, EPI and CAP groups revealed the highest improvement in kidney function, while the Egyptian, Chinese green tea leaves and POL groups showed a significant enhancement in hepato-billiary system. All the studied groups revealed a significant improvement in cardiac function. Moreover, MEST relative expression increased in HFR group and showed a significant down-regulation in all green tea groups but EPI revealed the lowest relative expression in MEST which suggested its effect on improving obesity in rats.</span>https://mvmj.journals.ekb.eg/article_127986_b49468fe4692011d06ab5003a1d5b68d.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212THE RECENT ELECTRON MICROSCOPY APPLICATIONS (ELECTRON TOMOGRAPHY)58759012798710.21608/mvmj.2017.127987ENMahmoud BadranShoeibAnatomy & Embryology, Department Faculty of Veterinary Medicine, Mansoura University.Journal Article20170912<span>Electron tomography is a new technique for obtaining detailed 3D structures of both biological and non-biological samples relevant to the life and biomedical sciences. Electron tomography is an extension of the traditional transmission electron microscopy. Different protocols concerning the 3D electron tomography, Conventional TEM, Immuno-electron microscopy and cryoEM will be covered. With these methods, relatively large volumes of resinembedded biological structures can be analyzed at resolutions of a few nm within a reasonable expenditure of time. Therefore, this work could support the collaborative research activities of many investigators from various departments in the faculty of veterinary medicine whose work has already had a major impact in the areas of biological, life and physical sciences.</span>https://mvmj.journals.ekb.eg/article_127987_d308da95343f44cadc0037157ee78876.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120171212HISTOPATHOLOGICAL ALTERATIONS IN INTESTINE OF MICE INFECTED WITH OF SCHISTOSOMA MANSONI58158512798910.21608/mvmj.2017.127989ENMohamed FHamedLecturer of pathology, pathology department, Mansoura University, Egypt, 35516Moustafa A.Al-ArabyLecturer of parasitology, parasitology department, Mansoura University, Egypt, 35516Journal Article20170912<span>Schistosomiasis remains an important health problem in many tropical countries and developing countries. It has serious pathological lesions in different tissues particularly liver. The aim of this study is to demonstrate histopathological alterations in intestine of mice infected with S. mansoni as model for human schistosomiasis. Twenty mice were infected by tail immersion technique (80±10 cercariae per mouse of Egyptian strain of S. mansoni). Animals were clinically observed for 7 weeks and then they were sacrificed. Gross and histological examination was done, in addition to Masson trichrome and scoring of fibrosis by image J analysis were performed. The results revealed severe weight loss of infected mice compared to control one, large number of neutrophiles in granuloma of S mansoni in intestinal submucosa, extensive fibrosis, about 12% from total area of intestine, that penetrate deeply into muscle layer. it was concluded that S. mansoni caused pyogranulomatous enteritis and extensive fibrosis that extended deeply into muscle layer of intestine.</span>https://mvmj.journals.ekb.eg/article_127989_c72b2535d89c2b17a01795152f5c0b5b.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120170601VIROLOGICAL STUDIES ON FOWL POX VIRUS (FPV) ISOLATED FROM CHICKENS IN DAKAHLIA AND DAMIETTA GOVERNORATES, EGYPT31732813041310.21608/mvmj.2017.130413ENAlyEl-KenwayDepartment of Virology, Faculty of eterinary Medicine, Mansoura University, Mansoura, EgyptOEI-BohySaharAbd El RahmanJournal Article20170510In the present study, 10 pooled samples including nodules and diphtheritic membranes were collected front vaccinated and non-vaccinated chicken flocks at Dakahlia and Damietta governorates, Egypt during the period from June 2016 to August 2016. Trials for isolation of suspected virus from the collected samples were carried out via choriallantoic membranes (CAMs) of 10 days old embryonated chicken eggs (ECEs), collected from hens free from Fowl pox virus (FPV). Three serial egg passages were carried out for each sample. The isolated virus from field and 3rd egg passaged samples was identified by agar gel precipitation test (AGPT), indirect fluorescent antibody test (IFAT) and histopathological examination. Hyperimmune serum was prepared against standard FPV vaccine in rabbit. The identified virus was confirmed by polymerase chain reaction (PCR). The results of virus isolation revealed that out of 10 pooled samples, 7 samples were positive results after 1st passage, 8 samples were positive results after 2nd passage and 10 samples were positive results after the 3'd passage. The percentage of the positive results for field samples identified by AGPT and IFAT were 90% (9 out of 10 samples) and 100% (all 10 samples) respectively, while after 3rd egg passage was 50% (5 out of 10 samples) by AGPT and 100%) (all samples) by IFAT. The results indicate that IFAT and AGPT could be used for virus identification., but IFAT is more sensitive. Histopathological examination revealed specific eosinophilic intracytoplasmic inclusion bodies of FPV. PCR for field samples and the 3rd egg passages gave positive results in all samples targeting P4b gene at 578 bp, so PCR technique is more accurate and sensitive.https://mvmj.journals.ekb.eg/article_130413_c799d68f3ef8da3ee5888a13cebf1322.pdfMansoura University, Faculty of Veterinary MedicineMansoura Veterinary Medical Journal1110-721918120170601POSTNATAL TOXIC EFFECTS OF FENITROTHION AND LEAD ACETATE IN ALBINO RATS14115819017910.21608/mvmj.2017.190179ENMamdouhAbouelmagdForensic Medicine and Toxicology Department, Fac. Vet. Med., Mansura University, EgyptFathySeleemForensic Medicine and Toxicology Department, Fac. Vet. Med., Mansura University, EgyptMAbd-elgawadFatmaAbdelhamidMohamedHamedAbdulrahmanAljaliForensic Medicine and Toxicology Department, Fac. Vet. Med., Omar AL- Mukhtar University, LibyaJournal Article20170319To investigate the toxic effects of fenitrothion and lead acetate on dams and their pups during lactation period. Twenty four female pregnant albino rats were divided into four groups, first group as control, second group received orally (80 mg/kg B.wt fenitrothion), third group received orally(60 mg/kg B.wt lead acetate), fourth group received orally (80 mg/kg fenitrothion B.wt plus 60 mg/kg B.wt lead acetate). The results indicated that decrease in body weight of pups for all treated groups compared to control group. The activity of ALT, AST and GGT increased in mothers serum, increased levels of creatinine and urea in mothers serum. Increased in oxidative stress, indicated by increased the level of MDA of liver homogenate for mothers and their pups, and decrease in the antioxidant enzymes, activities (GSH, GST and SOD) in liver homogenate for mothers and pups. Decrease ChE activity in mothers serum and its brain homogenate for mothers and pups. This result consistent histopathological changes were found in the liver, kidney, spleen, pancreas and brain of mothers and liver, kidney, spleen and brain of pups. The results suggested that the transfer of fenitrothion and lead acetate through the mother milk has resulted in oxidative stress and biochemical and histopathological alterations in the suckling pups. Also the combination between lead acetate and fenitrothion consider synergistic effects either on dams or pups.