%0 Journal Article %T Pathotyping of Field Identified Newcastle Disease Isolated from Quail in Egypt %J Mansoura Veterinary Medical Journal %I Mansoura University, Faculty of Veterinary Medicine %Z 1110-7219 %A Lebdah, Mohamed %A Abdelrahman, Sahar %A Awad, Naglaa %A karam, Reham %A Attia, Ahmed %A Ibrahim El bagoury, Mohamed %D 2022 %\ 06/06/2022 %V %N %P - %! Pathotyping of Field Identified Newcastle Disease Isolated from Quail in Egypt %K NDV %K Quail %K real time PCR %K Pathotyping %R 10.21608/mvmj.2022.127681.1109 %X The extreme population growth in developing countries, including Egypt, requires increased and alternative sources of animal proteins. Quails are excellent source. Newcastle disease (ND) causes substantial economic losses and represents perpetual threat to poultry species in Egypt and all over the world. limited data indicates the clinico-pathological features of NDV infection in quails as compared to conventional poultry species. To our knowledge, this work is one of the first studies illustrating the isolation and identification of ND from naturally infected quail farms. Isolation of Newcastle disease virus (NDV) was done using SPF-ECEs for three successive passages via allantoic cavity at 10-days old, while molecular identification was done first by real-time PCR to exclude H5 and H9, then conventional PCR targeting NDV was done. Pathogenicity of one selected isolate was done using ICPI and MDT. Two allantoic fluids out of eleven fluids were positive for avian influenza H5 subtype by real-time PCR, while all allantoic fluids were negative for H9 influenza virus.Allantoic fluids examined by conventional PCR showed five positive samples for NDV targeting the F gene. Pathogenicity indices (intracerebral pathogenicity index (ICPI) and mean death time (MDT) were 1.25 and 77, respectively, indicating that the tested isolate is mesogenic in pathogenicity. %U